The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.
Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
Protein modification; protein sumoylation.
Belongs to the PIAS family. Contains 1 PINIT domain. Contains 1 SAP domain. Contains 1 SP-RING-type zinc finger.
The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
Nucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
Protein inhibitor of activated STAT protein 4 antibody
Protein inhibitor of activated STAT protein gamma antibody
Protein inhibitor of activated STAT protein PIASy antibody
Zinc finger MIZ type containing 6 antibody
ZMIZ 6 antibody
Western blot - Anti-E3 SUMO-protein ligase PIAS4 antibody (ab13795)
Western blot analysis of PIAS gamma in Jurkat cell lysate using ab13795 at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence - Anti-E3 SUMO-protein ligase PIAS4 antibody (ab13795)This image is courtesy of an anonymous Abreview
ab13795 at 1/50 staining human fibroblasts by ICC/IF. The cells were fixed with formaldehyde and blocked with serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.
Nath AK et al. Proteomic-based detection of a protein cluster dysregulated during cardiovascular development identifies biomarkers of congenital heart defects. PLoS One4:e4221 (2009).
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