Product nameAnti-E3 SUMO-protein ligase PIAS4/PIASy antibody [10-24.6]
See all E3 SUMO-protein ligase PIAS4/PIASy primary antibodies
DescriptionMouse monoclonal [10-24.6] to E3 SUMO-protein ligase PIAS4/PIASy
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant fragment (His-tag) corresponding to Human E3 SUMO-protein ligase PIAS4/PIASy aa 2-473.
AAELVEAKN MVMSFRVSDL QMLLGFVGRS KSGLKHELVT RALQLVQFDC SPELFKKIKE LYETRYAKKN SEPAPQPHRP LDPLTMHSTY DRAGAVPRTP LAGPNIDYPV LYGKYLNGLG RLPAKTLKPE VRLVKLPFFN MLDELLKPTE LVPQNNEKLQ ESPCIFALTP RQVELIRNSR ELQPGVKAVQ VVLRICYSDT SCPQEDQYPP NIAVKVNHSY CSVPGYYPSN KPGVEPKRPC RPINLTHLMY LSSATNRITV TWGNYGKSYS VALYLVRQLT SSELLQRLKT IGVKHPELCK ALVKEKLRLD PDSEIATTGV RVSLICPLVK MRLSVPCRAE TCAHLQCFDA VFYLQMNEKK PTWMCPVCDK PAPYDQLIID GLLSKILSEC EDADEIEYLV DGSWCPIRAE KERSCSPQGA ILVLGPSDAN GLLPAPSVNG SGALGSTGGG GPVGSMENGK PGADVVDLTL DSS
Database link: Q8N2W9
- 293T and LINCap cell lysates; 293T cells; Myc tagged E3 SUMO-protein ligase PIAS4/PIASy transfected 293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.04% Sodium azide
Constituents: 89% PBS, 10% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab211625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionFunctions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.
Tissue specificityHighly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
PathwayProtein modification; protein sumoylation.
Sequence similaritiesBelongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger.
DomainThe LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
modificationsSumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
Cellular localizationNucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
- Information by UniProt
- E3 SUMO protein ligase PIAS4 antibody
- E3 SUMO-protein ligase PIAS4 antibody
- FLJ12419 antibody
All lanes : Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody [10-24.6] (ab211625) at 1/2500 dilution
Lane 1 : 293T cell lysate
Lane 2 : LINCap cell lysate
Immunofluorescent analysis of 293T cells labeling E3 SUMO-protein ligase PIAS4/PIASy using ab21162 at 1/250 dilution. An Alexa Fluor 488 conjugated sheep anti mouse was used as secondary antibody.
Lanes 1-4 : anti Myc antibody
Lanes 6-10 : Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody [10-24.6] (ab211625) at 1/2500 dilution
Lanes 1 & 6 : Untransfected 293T cell lysate
Lanes 2 & 7 : 293T cell lysate transfected with Myc tagged PIAS1 protein
Lanes 3 & 8 : 293T cell lysate transfected with Myc tagged PIASXbeta protein
Lanes 4 & 9 : 293T cell lysate transfected with Myc tagged PIAS3 protein
Lanes 5 & 10 : 293T cell lysate transfected with Myc tagged E3 SUMO-protein ligase PIAS4 protein
This product has been referenced in:
- Wang R et al. Reversible regulation of ORC2 SUMOylation by PIAS4 and SENP2. Oncotarget 8:70142-70155 (2017). Read more (PubMed: 29050267) »
- Huang W et al. SUMO E3 Ligase PIASy Mediates High Glucose-Induced Activation of NF-?B Inflammatory Signaling in Rat Mesangial Cells. Mediators Inflamm 2017:1685194 (2017). Read more (PubMed: 29038616) »