Overview

  • Product name

    Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody
    See all E3 SUMO-protein ligase PIAS4/PIASy primary antibodies
  • Description

    Rabbit polyclonal to E3 SUMO-protein ligase PIAS4/PIASy
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human E3 SUMO-protein ligase PIAS4/PIASy aa 441-494.

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, Molt4 and Raji cell lysates; DLD1 Xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab137500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.

Target

  • Function

    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Promotes PARK7 sumoylation. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations.
  • Tissue specificity

    Highly expressed in testis and, at lower levels, in spleen, prostate, ovary, colon and peripheral blood leukocytes.
  • Pathway

    Protein modification; protein sumoylation.
  • Sequence similarities

    Belongs to the PIAS family.
    Contains 1 PINIT domain.
    Contains 1 SAP domain.
    Contains 1 SP-RING-type zinc finger.
  • Domain

    The LXXLL motif is a coregulator signature that is essential for transcriptional corepression.
  • Post-translational
    modifications

    Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activitation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate.
  • Cellular localization

    Nucleus > PML body. Colocalizes with SUMO1 and TCF7L2/TCF4 and LEF1 in a subset of PML (promyelocytic leukemia) nuclear bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 SUMO protein ligase PIAS4 antibody
    • E3 SUMO-protein ligase PIAS4 antibody
    • FLJ12419 antibody
    • MGC35296 antibody
    • PAIASgamma antibody
    • PIAS 4 antibody
    • PIAS gamma antibody
    • PIAS-gamma antibody
    • Pias4 antibody
    • PIAS4_HUMAN antibody
    • PIASG antibody
    • PIASgamma antibody
    • PIASy antibody
    • Protein inhibitor of activated STAT 4 antibody
    • Protein inhibitor of activated STAT protein 4 antibody
    • Protein inhibitor of activated STAT protein gamma antibody
    • Protein inhibitor of activated STAT protein PIASy antibody
    • Zinc finger MIZ type containing 6 antibody
    • ZMIZ 6 antibody
    • ZMIZ6 antibody
    see all

Images

  • All lanes : Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (ab137500) at 1/1000 dilution

    Lane 1 : HeLaS3 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 56 kDa



    7.5% SDS PAGE
  • Confocal immunofluorescence analysis analysis of paraformaldehyde-fixed HeLa cell labelling E3 SUMO-protein ligase PIAS4/PIASy with ab137500 (green) at a 1/500 dilution. Alpha-tubulin filaments were labeled with an Alpha-tubulin antibody (red) at a 1/2000 dilution.

  • Immunohistochemical analysis of paraffin embedded DLD1 Xenograft labelling E3 SUMO-protein ligase PIAS4/PIASy with ab137500 at a 1/100 dilution.

References

This product has been referenced in:

  • Heras G  et al. Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification. J Mol Cell Biol 11:356-370 (2019). Read more (PubMed: 29868881) »
See 1 Publication for this product

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