Key features and details
- Rabbit polyclonal to E3 ubiquitin-protein ligase MUL1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-E3 ubiquitin-protein ligase MUL1 antibody
See all E3 ubiquitin-protein ligase MUL1 primary antibodies
DescriptionRabbit polyclonal to E3 ubiquitin-protein ligase MUL1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to a region within amino acids 1-352 of Human E3 ubiquitin-protein ligase MUL1 (UniProt Q969V5).
- 293T, A431 and Raji cell lysates; A431 cells.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 40 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionExhibits weak E3 ubiquitin-protein ligase activity, but preferentially acts as a SUMO E3 ligase at physiological concentrations. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Tissue specificityWidely expressed with highest levels in the heart, skeletal muscle, placenta, kidney and liver. Barely detectable in colon and thymus.
PathwayProtein modification; protein ubiquitination.
Protein modification; protein sumoylation.
Sequence similaritiesContains 1 RING-type zinc finger.
DomainThe zinc finger domain is required for E3 ligase activity.
Cellular localizationMitochondrion outer membrane. Peroxisome. Transported in mitochondrion-derived vesicles from the mitochondrion to the peroxisome.
- Information by UniProt
- 0610009K11Rik antibody
- AV000801 antibody
- C1orf166 antibody
Confocal immunofluorescent analysis of methanol-fixed A431 cells labeling E3 ubiquitin-protein ligase MUL1 with ab155511 at 1/500 dilution (Green). Alpha-tubulin filaments are labeled red.
Anti-E3 ubiquitin-protein ligase MUL1 antibody (ab155511) at 1/2000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 40 kDa
10% SDS PAGE
ab155511 has not yet been referenced specifically in any publications.