Overview

  • Product name

    Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241]
    See all E3 ubiquitin-protein ligase MUL1 primary antibodies
  • Description

    Rabbit monoclonal [EPR20241] to E3 ubiquitin-protein ligase MUL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human E3 ubiquitin-protein ligase MUL1 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q969V5

  • Positive control

    • WB: HEK-293, HeLa, PC-3 and A431 whole cell lysates; Human fetal heart lysate. IP: HeLa whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

Target

  • Function

    Exhibits weak E3 ubiquitin-protein ligase activity, but preferentially acts as a SUMO E3 ligase at physiological concentrations. Plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
  • Tissue specificity

    Widely expressed with highest levels in the heart, skeletal muscle, placenta, kidney and liver. Barely detectable in colon and thymus.
  • Pathway

    Protein modification; protein ubiquitination.
    Protein modification; protein sumoylation.
  • Sequence similarities

    Contains 1 RING-type zinc finger.
  • Domain

    The zinc finger domain is required for E3 ligase activity.
  • Cellular localization

    Mitochondrion outer membrane. Peroxisome. Transported in mitochondrion-derived vesicles from the mitochondrion to the peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • 0610009K11Rik antibody
    • AV000801 antibody
    • C1orf166 antibody
    • Chromosome 1 open reading frame 166 antibody
    • E3 SUMO-protein ligase MUL1 antibody
    • E3 ubiquitin ligase antibody
    • E3 ubiquitin protein ligase MUL1 antibody
    • E3 ubiquitin-protein ligase MUL1 antibody
    • FLJ12875 antibody
    • GIDE antibody
    • Growth inhibition and death E3 ligase antibody
    • MAPL antibody
    • Mitochondrial anchored protein ligase antibody
    • mitochondrial E3 ubiquitin ligase 1 antibody
    • mitochondrial E3 ubiquitin protein ligase 1 antibody
    • Mitochondrial ubiquitin ligase activator of NFKB 1 antibody
    • Mitochondrial-anchored protein ligase antibody
    • MUL1 antibody
    • MUL1_HUMAN antibody
    • MULAN antibody
    • Putative NF kappa B activating protein 266 antibody
    • Putative NF-kappa-B-activating protein 266 antibody
    • RGD1309944 antibody
    • RING finger protein 218 antibody
    • RNF218 antibody
    • RP11-401M16.2 antibody
    • RP23-25C1.10-002 antibody
    see all

Images

  • All lanes : Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] (ab209263) at 1/1000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 4 : Human fetal heart lysate
    Lane 5 : Human thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa


    Exposure time: 15 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Negative control: Human thymus (PMID: 18591963).

  • Anti-E3 ubiquitin-protein ligase MUL1 antibody [EPR20241] (ab209263) at 1/1000 dilution + A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • E3 ubiquitin-protein ligase MUL1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab209263 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab209263 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate, 10 µg (Input).

    Lane 2: ab209263 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209263 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

References

ab209263 has not yet been referenced specifically in any publications.

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