Overview

  • Product name

  • Description

    Rabbit polyclonal to E4F1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residue 275 and 325 of human E4F1

  • Positive control

    • HeLa, 293T, and MCF7 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab70615 was affinity purified using an epitope specific to E4F1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 84 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      E4F1 is a zinc finger transcription factor that inhibits cell proliferation. E4F1 interacts with Bmi1 in hematopoietic cells and is important for the regulation of stem cell proliferation.
    • Cellular localization

      Nuclear
    • Database links

    • Alternative names

      • E4F antibody
      • E4F transcription factor 1 antibody
      • E4F transcription factor 1, isoform CRA_b antibody
      • p120E4F antibody
      • P120E4F transcription factor antibody
      • p50E4F antibody
      • Putative E3 ubiquitin-protein ligase E4F1 antibody
      • RING type E3 ubiquitin transferase E4F1 antibody
      • Transcription factor E4F1 antibody
      see all

    Images

    • All lanes : Anti-E4F1 antibody (ab70615) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate
      Lane 2 : 293T whole cell lysate
      Lane 3 : MCF7 whole cell lysate

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 84 kDa
      Observed band size: 120 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes
    • Detection of Human E4F1 by Western Blot of Immunprecipitate. ab70615 at 1µg/ml staining E4F1 in MCF7 whole cell lysates immunoprecipitated using ab70615 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes

    References

    This product has been referenced in:

    • Gui Z  et al. Poly(ADP-ribose) polymerase-1 promotes expression of miR-155 by the up-regulation of methyl-CpG binding domain protein 2 in TK6 cells exposed to hydroquinone. Toxicol In Vitro 55:51-57 (2019). Read more (PubMed: 30448557) »
    See 1 Publication for this product

    Customer reviews and Q&As

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