Key features and details
- Rabbit polyclonal to E4F1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-E4F1 antibody
See all E4F1 primary antibodies
DescriptionRabbit polyclonal to E4F1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to a region between residue 275 and 325 of human E4F1
- HeLa, 293T, and MCF7 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab70615 was affinity purified using an epitope specific to E4F1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab70615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 84 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceE4F1 is a zinc finger transcription factor that inhibits cell proliferation. E4F1 interacts with Bmi1 in hematopoietic cells and is important for the regulation of stem cell proliferation.
- E4F antibody
- E4F transcription factor 1 antibody
- E4F transcription factor 1, isoform CRA_b antibody
All lanes : Anti-E4F1 antibody (ab70615) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : MCF7 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 84 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Detection of Human E4F1 by Western Blot of Immunprecipitate. ab70615 at 1µg/ml staining E4F1 in MCF7 whole cell lysates immunoprecipitated using ab70615 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes
ab70615 has been referenced in 1 publication.
- Gui Z et al. Poly(ADP-ribose) polymerase-1 promotes expression of miR-155 by the up-regulation of methyl-CpG binding domain protein 2 in TK6 cells exposed to hydroquinone. Toxicol In Vitro 55:51-57 (2019). PubMed: 30448557