Anti-eNOS (phospho S632) antibody [M232] (ab76199)
Key features and details
- Mouse monoclonal [M232] to eNOS (phospho S632)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-eNOS (phospho S632) antibody [M232]
See all eNOS primary antibodies -
Description
Mouse monoclonal [M232] to eNOS (phospho S632) -
Host species
Mouse -
Specificity
ab76199 is not expected to react with other NOS family members due to low homology. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Mouse eNOS (phospho S632).
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Positive control
- Human umbilical vein endothelial cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M232 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500. Detects a band of approximately 140, 120 kDa (predicted molecular weight: 133 kDa).
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Notes |
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WB
1/500. Detects a band of approximately 140, 120 kDa (predicted molecular weight: 133 kDa). |
Target
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Function
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. -
Tissue specificity
Platelets, placenta, liver and kidney. -
Involvement in disease
Variation in NOS3 seem to be associated with susceptibility to coronary spasm. -
Sequence similarities
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
Post-translational
modificationsPhosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. -
Cellular localization
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity. - Information by UniProt
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Database links
- Entrez Gene: 4846 Human
- Entrez Gene: 24600 Rat
- Omim: 163729 Human
- SwissProt: P29474 Human
- SwissProt: Q62600 Rat
- Unigene: 647092 Human
- Unigene: 44265 Rat
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Alternative names
- cNOS antibody
- Constitutive NOS antibody
- EC NOS antibody
see all
Images
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All lanes : Anti-eNOS (phospho S632) antibody [M232] (ab76199) at 1/500 dilution
Lane 1 : human umbilical vein endothelial
cells, untreated
Lane 2 : human umbilical vein endothelial
cells, treated with lambda phosphatase
Predicted band size: 133 kDa
Observed band size: 120,140 kDa why is the actual band size different from the predicted?
The 120 kDa band may be a truncated form of eNOS.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab76199 has been referenced in 15 publications.
- Guan Y & Wang X Salvianic Acid A Regulates High-Glucose-Treated Endothelial Progenitor Cell Dysfunction via the AKT/Endothelial Nitric Oxide Synthase (eNOS) Pathway. Med Sci Monit 27:e928153 (2021). PubMed: 33770068
- Cheng X et al. Inhibition of LOXL1-AS1 alleviates oxidative low-density lipoprotein induced angiogenesis via downregulation of miR-590-5p mediated KLF6/VEGF signaling pathway. Cell Cycle 20:1663-1680 (2021). PubMed: 34334119
- Ye P et al. Endothelial Agrin Is Dispensable for Normal and Tumor Angiogenesis. Front Cardiovasc Med 8:810477 (2021). PubMed: 35174224
- Xu J et al. Knockdown of Long Noncoding RNA (lncRNA) AK094457 Relieved Angiotensin II Induced Vascular Endothelial Cell Injury. Med Sci Monit 26:e919854 (2020). PubMed: 32027625
- Xu H et al. GATA4 protects against hyperglycemia-induced endothelial dysfunction by regulating NOX4 transcription. Mol Med Rep 17:1485-1492 (2018). PubMed: 29138836