Key features and details
- Chicken polyclonal to EAAT1
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgY
Product nameAnti-EAAT1 antibody
See all EAAT1 primary antibodies
DescriptionChicken polyclonal to EAAT1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: Rat pup PND2-6 - minimal cortex brain lysate. ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235203 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Predicted molecular weight: 59 kDa.|
|ICC/IF||Use a concentration of 15 µg/ml.|
FunctionTransports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Tissue specificityHighly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
Involvement in diseaseDefects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
Sequence similaritiesBelongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
- Information by UniProt
- EA6 antibody
- EAA1_HUMAN antibody
- EAAT1 antibody
4% PFA fixed, 0.15% Triton X-100 permeabilized post-natal rat pup (PND1) heterogeneous brain cells* labeling EAAT1 using ab235203 at 15 µg/ml, O/N at +4°C (green) followed by Goat Anti-Chicken IgG DyLight™ 488 conjugated preadsorbed at 5 µg/ml for 1 h at RT, in ICC/IF. Nuclear probe is visualized in blue (DAPI).
*UniprotID Expression Tissue Specificity and Subcellular Localization, as well as additional information found in Human Protein Atlas.
Expected localization: Cell membrane (mitochondria, nucleus)
Observed localization: punctate staining indicates mitochondrial detection**
**Staining as confirmed in Gensat for Slc1a3 (high affinity glial glutamate transporter): http://www.gensat.org/imagenavigator.jsp?imageID=10581
All lanes : Anti-EAAT1 antibody (ab235203) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 20 µg
Lane 2 : Adult mouse whole liver lysate at 20 µg
Lane 3 : Rat pup PND2-6 - minimal cortex brain lysate* at 20 µg
Lane 4 : Rat pup PND2-6 - minimal cortex brain lysate* at 40 µg
All lanes : Goat Anti-Chicken IgY H&L (HRP) preadsorbed (ab7118) at 1/40000 dilution
Predicted band size: 59 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 60 seconds
*UniprotID Expression Tissue Specificity: Detected in brain and cerebellum (PubMed:1279699, PubMed:8387171). Both isoform EAAT1/GLAST-1 and EAAT1/GLAST-1A are expressed in bone and brain (PubMed:11086157). In brain isoform EAAT1/GLAST-1 is highly enriched in the Purkinje cell layer in cerebellum (PubMed:11086157).
Minimal cortex defined: minimal cortex brain samples have had a portion of the cortex removed, with 1/3 cortex, cerebellum and other brain components present in the final lysate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235203 has not yet been referenced specifically in any publications.