Overview

  • Product name

  • Description

    Chicken polyclonal to EAAT1
  • Host species

    Chicken
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Rat pup PND2-6 - minimal cortex brain lysate. ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab235203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 59 kDa.
ICC/IF Use a concentration of 15 µg/ml.

Target

  • Function

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • Tissue specificity

    Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
  • Involvement in disease

    Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
  • Sequence similarities

    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EA6 antibody
    • EAA1_HUMAN antibody
    • EAAT1 antibody
    • Excitatory amino acid transporter 1 antibody
    • FLJ25094 antibody
    • GLAST antibody
    • GLAST-1 antibody
    • GLAST1 antibody
    • Glial high affinity glutamate transporter antibody
    • glutamate/aspartate transporter, high affinity, sodium-dependent antibody
    • High affinity neuronal glutamate transporter antibody
    • Slc1a3 antibody
    • Sodium dependent glutamate/aspartate transporter antibody
    • Sodium-dependent glutamate/aspartate transporter 1 antibody
    • Solute carrier family 1 (glial high affinity glutamate transporter) member 3 antibody
    • Solute carrier family 1 member 3 antibody
    see all

Images

  • 4% PFA fixed, 0.15% Triton X-100 permeabilized post-natal rat pup (PND1) heterogeneous brain cells* labeling EAAT1 using ab235203 at 15 µg/ml, O/N at +4°C (green) followed by Goat Anti-Chicken IgG DyLight™ 488 conjugated preadsorbed at 5 µg/ml for 1 h at RT, in ICC/IF. Nuclear probe is visualized in blue (DAPI).

    *UniprotID Expression Tissue Specificity and Subcellular Localization, as well as additional information found in Human Protein Atlas.

    Expected localization:  Cell membrane (mitochondria, nucleus)
    Observed localization: punctate staining indicates mitochondrial detection**

    **Staining as confirmed in Gensat for Slc1a3 (high affinity glial glutamate transporter): http://www.gensat.org/imagenavigator.jsp?imageID=10581

  • All lanes : Anti-EAAT1 antibody (ab235203) at 1/500 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 20 µg
    Lane 2 : Adult mouse whole liver lysate at 20 µg
    Lane 3 : Rat pup PND2-6 - minimal cortex brain lysate* at 20 µg
    Lane 4 : Rat pup PND2-6 - minimal cortex brain lysate* at 40 µg

    Secondary
    All lanes : Goat Anti-Chicken IgY H&L (HRP) preadsorbed (ab7118) at 1/40000 dilution

    Predicted band size: 59 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?


    Exposure time: 60 seconds


    *UniprotID Expression Tissue Specificity: Detected in brain and cerebellum (PubMed:1279699, PubMed:8387171). Both isoform EAAT1/GLAST-1 and EAAT1/GLAST-1A are expressed in bone and brain (PubMed:11086157). In brain isoform EAAT1/GLAST-1 is highly enriched in the Purkinje cell layer in cerebellum (PubMed:11086157). 

    Minimal cortex defined: minimal cortex brain samples have had a portion of the cortex removed, with 1/3 cortex, cerebellum and other brain components present in the final lysate.

References

ab235203 has not yet been referenced specifically in any publications.

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