Anti-EAAT1 antibody (ab416)
Key features and details
- Rabbit polyclonal to EAAT1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EAAT1 antibody
See all EAAT1 primary antibodies -
Description
Rabbit polyclonal to EAAT1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat
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Immunogen
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General notes
Glutamate-aspartate transporter is also known as GLAST.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1. Lot numbers other than GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/200 - 1/5000. Predicted molecular weight: 60 kDa.
|
| IHC-P | (3) |
1/50 - 1/500.
|
| Notes |
|---|
|
WB
1/200 - 1/5000. Predicted molecular weight: 60 kDa. |
|
IHC-P
1/50 - 1/500. |
Target
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Function
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. -
Tissue specificity
Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia. -
Involvement in disease
Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia. -
Sequence similarities
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6507 Human
- Entrez Gene: 29483 Rat
- Omim: 600111 Human
- SwissProt: P43003 Human
- SwissProt: P24942 Rat
- Unigene: 481918 Human
- Unigene: 34134 Rat
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Alternative names
- EA6 antibody
- EAA1_HUMAN antibody
- EAAT1 antibody
see all
Images
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Western blot - Anti-EAAT1 antibody (ab416)Anti-EAAT1 antibody (ab416) + Rat brain cortex
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 150 kDa (possible dimer) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody (ab416)ab416 (1:500) staining EAAT1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane cells in the purkinje glial region .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (86)
ab416 has been referenced in 86 publications.
- Limbad C et al. Astrocyte senescence promotes glutamate toxicity in cortical neurons. PLoS One 15:e0227887 (2020). PubMed: 31945125
- Zhang RS et al. Latrophilin-2 and latrophilin-3 are redundantly essential for parallel-fiber synapse function in cerebellum. Elife 9:N/A (2020). PubMed: 32202499
- Silva-Adaya D et al. Early Neurotoxic Effects of Inorganic Arsenic Modulate Cortical GSH Levels Associated With the Activation of the Nrf2 and NF?B Pathways, Expression of Amino Acid Transporters and NMDA Receptors and the Production of Hydrogen Sulfide. Front Cell Neurosci 14:17 (2020). PubMed: 32194376
- Peterson AR & Binder DK Regulation of Synaptosomal GLT-1 and GLAST during Epileptogenesis. Neuroscience 411:185-201 (2019). PubMed: 31158434
- Janssen K et al. Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling. Methods Mol Biol 1994:31-39 (2019). PubMed: 31124102