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  1. Link

    eaat1-antibody-ab416.pdf

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Neuroscience Neurotransmitter Amino Acids Glutamate
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Anti-EAAT1 antibody (ab416)

  • Datasheet
  • SDS
Reviews (12)Q&A (22)References (111)

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Western blot - Anti-EAAT1 antibody (ab416)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody (ab416)

Key features and details

  • Rabbit polyclonal to EAAT1
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-EAAT1 antibody
    See all EAAT1 primary antibodies
  • Description

    Rabbit polyclonal to EAAT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat EAAT1 (C terminal). Corresponding to 20 residues from the C-terminal.
    Database link: P24942
    (Peptide available as ab127026)

  • General notes

    Glutamate-aspartate transporter is also known as GLAST.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1,  GR320843-2, GR320843-3, GR313192-1, GR320843-1. Lot numbers other than GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1,  GR320843-2, GR320843-3, GR313192-1, GR320843-1 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Aspartate
    • Neuroscience
    • Neurotransmitter
    • Transporters
    • Glutamate
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Stem Cells
    • Neural Stem Cells
    • Surface Molecules
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/200 - 1/5000. Predicted molecular weight: 60 kDa.
IHC-P (3)
1/50 - 1/500.
Notes
WB
1/200 - 1/5000. Predicted molecular weight: 60 kDa.
IHC-P
1/50 - 1/500.

Target

  • Function

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • Tissue specificity

    Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
  • Involvement in disease

    Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
  • Sequence similarities

    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P43003 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6507 Human
    • Entrez Gene: 29483 Rat
    • Omim: 600111 Human
    • SwissProt: P43003 Human
    • SwissProt: P24942 Rat
    • Unigene: 481918 Human
    • Unigene: 34134 Rat
    • Alternative names

      • EA6 antibody
      • EAA1_HUMAN antibody
      • EAAT1 antibody
      • Excitatory amino acid transporter 1 antibody
      • FLJ25094 antibody
      • GLAST antibody
      • GLAST-1 antibody
      • GLAST1 antibody
      • Glial high affinity glutamate transporter antibody
      • glutamate/aspartate transporter, high affinity, sodium-dependent antibody
      • High affinity neuronal glutamate transporter antibody
      • Slc1a3 antibody
      • Sodium dependent glutamate/aspartate transporter antibody
      • Sodium-dependent glutamate/aspartate transporter 1 antibody
      • Solute carrier family 1 (glial high affinity glutamate transporter) member 3 antibody
      • Solute carrier family 1 member 3 antibody
      see all

    Images

    • Western blot - Anti-EAAT1 antibody (ab416)
      Western blot - Anti-EAAT1 antibody (ab416)
      Anti-EAAT1 antibody (ab416) + Rat brain cortex

      Predicted band size: 60 kDa
      Observed band size: 60 kDa
      Additional bands at: 150 kDa (possible dimer)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody (ab416)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody (ab416)
      ab416 (1:500) staining EAAT1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane cells in the purkinje glial region .
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (111)

    Publishing research using ab416? Please let us know so that we can cite the reference in this datasheet.

    ab416 has been referenced in 111 publications.

    • Liu J  et al. Abnormal Glu/mGluR2/3/PI3K pathway in the hippocampal neurovascular unit leads to diabetes-related depression. Neural Regen Res 16:727-733 (2021). PubMed: 33063735
    • Hayashi H  et al. Effects of laminin-111 peptide coatings on rat neural stem/progenitor cell culture. Exp Cell Res 400:112440 (2021). PubMed: 33359470
    • Webber JL  et al. Axodendritic versus axosomatic cochlear efferent termination is determined by afferent type in a hierarchical logic of circuit formation. Sci Adv 7:N/A (2021). PubMed: 33523928
    • Kronschläger MT  et al. Lamina-specific properties of spinal astrocytes. Glia 69:1749-1766 (2021). PubMed: 33694249
    • Morcom L  et al. DCC regulates astroglial development essential for telencephalic morphogenesis and corpus callosum formation. Elife 10:N/A (2021). PubMed: 33871356
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 34 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EAAT1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (brain cortex)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    brain cortex
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 10 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EAAT1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain cortex)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    brain cortex
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 10 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EAAT1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (brain tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate ph 6
    Permeabilization
    No
    Specification
    brain tissue
    Blocking step
    Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
    Fixative
    Formaldehyde
    Read More

    Herr Dr. Markus Kipp

    Verified customer

    Submitted Sep 07 2018

    Immunohistochemistry free floating abreview for Anti-EAAT1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 16 2018

    Flow Cytometry abreview for Anti-EAAT1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Differentiated hNSCs)
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy
    Undifferentiated Stem Cells (white)
    Specification
    Differentiated hNSCs
    Preparation
    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

    Western blot abreview for Anti-EAAT1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 23 2015

    Immunohistochemistry (Frozen sections)

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Retina, Inner plexiform layer)
    Specification
    Retina, Inner plexiform layer
    Permeabilization
    Yes - 0.1% TritonX
    Fixative
    Paraformaldehyde
    Read More

    DR. Ryan Macdonald

    Verified customer

    Submitted Jun 05 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-EAAT1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Chicken Cell (retina)
    Specification
    retina
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton X-100 (0.02%) diluted in PBS
    Blocking step
    Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    MR. Chris Zelinka

    Verified customer

    Submitted Apr 23 2013

    Question

    This antibody is showing non-specific bands in WB.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 11 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement from a different lot.

    I apologize, but I misspoke on the phone. This item will be shipping from our UK office, so you can expect to receive it on April 16th once it transfers to our Boston office.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Apr 11 2012

    Question

    I have an enquiry that is virtually identical to one that was entered in the enquiry databast on Nov. 18, 2004. I want to use the antibody for the same application, to sort acutely dissociated mouse astrocytes. However, I wish to sort live cells for later tissue culture experiments, so it is important for me that the antibody recognizes an extracellular epitope. I have tried the antibody to GLT-1 (from Chemicon), a related protein. Abcam also makes an antibody to this protein (ab417) raised against a C-terminal synthetic peptide. Although the one worked beautifully in immunostaining, it did not work for my purposes since the peptide was likely to be in a cytoplasmic or transmembrane domain of the transporter protein. I am worried that the same might be true for ab416. I was wondering if you had received feedback from that particular enquiry or if it would be possible to put me in contact with the person who wrote it. I can be contacted at this email address. Also, if you have any structural information that would suggest the likely localization of the epitope, I would be very grateful to hear this information. Alternatively, would it be possible to obtain a sample of the antibody to test for the application of live cell sorting? I would be happy to share my results and protocols. Thanks so much for your time,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 30 2004

    Answer

    My sincere apologies for the delay, I have been trying to find out the epitope for ab416 and was unfortunately unsuccessful. The glutamate aspartate transporter GLAST is actually located both in the cytoplasm and at the membrane: it can be found localised closely to the membrane as it can be translocated into the membrane. I hope this information helps, please do not hesitate to contact us if you need further information,

    Read More

    Abcam Scientific Support

    Answered on Dec 22 2004

    1-10 of 34 Abreviews or Q&A

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