Anti-EAAT1 antibody (ab416)
- Datasheet
- References (52)
- Protocols
Overview
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Product nameAnti-EAAT1 antibody
See all EAAT1 primary antibodies -
DescriptionRabbit polyclonal to EAAT1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-FoFr, WB, ICC, Flow Cyt, IHC-P, ICC/IF, IHC-Frmore details
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Species reactivityReacts with: Mouse, Rat, Human, Zebrafish
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Immunogen
Synthetic peptide, corresponding to 20 residues from the C-terminus of rat EAAT1 (Peptide available as ab127026.)
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General notes
Glutamate-aspartate transporter is also known as GLAST.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 23rd June 2017. The following lots are still unpurified and still in stock as of 23rd June 2017 - GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1. Lot numbers other than GR3178513-1,GR3173220-1, GR3173220-2, GR293270-1, GR320843-2, GR320843-3, GR313192-1, GR320843-1 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferConstituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
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Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Related Products
Applications
Our Abpromise guarantee covers the use of ab416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 18714338 | |
| WB | 1/200 - 1/5000. Predicted molecular weight: 60 kDa.Can be blocked with EAAT1 peptide (ab127026). | |
| ICC | Use at an assay dependent concentration. | |
| Flow Cyt | 1/10 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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FunctionTransports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
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Tissue specificityHighly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
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Involvement in diseaseDefects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
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Sequence similaritiesBelongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
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Post-translational
modificationsGlycosylated. -
Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 6507 Human
- Entrez Gene: 20512 Mouse
- Entrez Gene: 29483 Rat
- Omim: 600111 Human
- SwissProt: P43003 Human
- SwissProt: P56564 Mouse
- SwissProt: P24942 Rat
- Unigene: 481918 Human
see all -
Alternative names
- EA6 antibody
- EAA1_HUMAN antibody
- EAAT1 antibody
see all
Images
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Western blot - Anti-EAAT1 antibody (ab416)Anti-EAAT1 antibody (ab416) + Rat brain cortex
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 150 kDa (possible dimer) -
Immunohistochemistry (Frozen sections) - Anti-EAAT1 antibody (ab416)This image is courtesy of an Abreview submitted by Dr. Ryan MacDonaldab416 staining zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5%BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab416 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. EAAT1 expression can be observed in the inner plexiform layer (in green).
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Flow Cytometry - Anti-EAAT1 antibody (ab416)Image courtesy of an anonymous AbReviewFlow cytometric analysis with ab416 at 1/300 dilution of differentiated human NSCs (green) and undifferentiated stem cells (white). Alexa Fluor© 488 goat anti-rabbit IgG, at 1/500 dilution was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody (ab416)This image is courtesy of an Abreview submitted by Chris Zelinkaab416 staining EAAT1 in Chicken retina cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.02% in PBS and blocked with 5% serum for 16 hours at 22°C. Samples were incubated with primary antibody (1/500 in PBS + 0.02% Triton) for 16 hours at 22°C. An undiluted Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-EAAT1 antibody (ab416)Image from Barcia C. et. al., PLoS One. 2008 Aug 20;3(8):e2977 (Fig 8C).ab416 at 1/100 dilution staining EAAT1 in mouse coronal tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Adult Sprague–Dawley rats were injected intraperitoneally with pentobarbitone and were transcardially perfused-fixed with heparinized saline and 4% paraformaldehyde in PBS. Brains were removed, were postfixed in 4% paraformaldehyde for 5h, and were stored in PBS. Sections of each brain were pretreated with citrate buffer for 30 min at 65°C to increase antigen retrieval and penetration of the antibodies into the tissues. Sections were permeabilized with 1% Triton X-100 for 5 min and blocked with 3% normal horse serum in 0.1 M PBS, pH 7.4, for 60 min and incubated at room temperature for 48h with primary antibodies. An Alexa Fluor® 488 conjugated anti rabbit was used as secondary at 1/1000 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody (ab416)ab416 (1:500) staining EAAT1 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane cells in the purkinje glial region .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-EAAT1 antibody (ab416)This image is courtesy of an anonymous Abreviewab416 staining EAAT1 in Rat Astrocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% Serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal(1/300) was used as the secondary antibody.
Datasheets and documents
Protocols
References
This product has been referenced in:
- Kia A et al. Astrocytes expressing ALS-linked mutant FUS induce motor neuron death through release of tumor necrosis factor-alpha. Glia 66:1016-1033 (2018). WB ; Mouse . Read more (PubMed: 29380416) »
- Johnson J et al. Valproate and sodium butyrate attenuate manganese-decreased locomotor activity and astrocytic glutamate transporters expression in mice. Neurotoxicology 64:230-239 (2018). Read more (PubMed: 28610743) »
