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  1. Link

    eaat1-antibody-epr12686-ab181036.pdf

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Neuroscience Neurotransmitter Amino Acids Glutamate
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RecombinantRabMAb

Recombinant Anti-EAAT1 antibody [EPR12686] (ab181036)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (6)

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Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Immunocytochemistry - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Immunocytochemistry - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Immunocytochemistry - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)
  • Anti-EAAT1 antibody [EPR12686] (ab181036)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12686] to EAAT1
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-EAAT1 antibody [EPR12686]
    See all EAAT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12686] to EAAT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Monkey
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain, rat brain and human cerebellum lysates. IHC-P: Human , Mouse and Rat cerebral cortex tissue sections. ICC: Mouse and rat primary neural / glia cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12686
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Aspartate
    • Neuroscience
    • Neurotransmitter
    • Transporters
    • Glutamate
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-EAAT1 antibody [EPR12686] - BSA and Azide free (ab240235)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/50.
IHC-P
1/50 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.
Notes
ICC
1/50.
IHC-P
1/50 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 59 kDa.

Target

  • Function

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • Tissue specificity

    Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
  • Involvement in disease

    Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
  • Sequence similarities

    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P43003 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6507 Human
    • Entrez Gene: 20512 Mouse
    • Entrez Gene: 29483 Rat
    • Omim: 600111 Human
    • SwissProt: P43003 Human
    • SwissProt: P56564 Mouse
    • SwissProt: P24942 Rat
    • Unigene: 481918 Human
    • Unigene: 204834 Mouse
    • Unigene: 34134 Rat
    see all
  • Alternative names

    • EA6 antibody
    • EAA1_HUMAN antibody
    • EAAT1 antibody
    • Excitatory amino acid transporter 1 antibody
    • FLJ25094 antibody
    • GLAST antibody
    • GLAST-1 antibody
    • GLAST1 antibody
    • Glial high affinity glutamate transporter antibody
    • glutamate/aspartate transporter, high affinity, sodium-dependent antibody
    • High affinity neuronal glutamate transporter antibody
    • Slc1a3 antibody
    • Sodium dependent glutamate/aspartate transporter antibody
    • Sodium-dependent glutamate/aspartate transporter 1 antibody
    • Solute carrier family 1 (glial high affinity glutamate transporter) member 3 antibody
    • Solute carrier family 1 member 3 antibody
    see all

Images

  • Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
    All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution

    Lane 1 : Mouse brain lysate boiled
    Lane 2 : Mouse brain lysate unboiled

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 59 kDa


    Exposure time: 10 seconds


    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    We recommend not to boil the samples after lysis to get desired WB results.

  • Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
    All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution

    Lane 1 : Mouse brain
    Lane 2 : Mouse hippocampus
    Lane 3 : Rat hippocampus
    Lane 4 : Rat brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 59 kDa
    Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

    ab181036 staining EAAT1 in mouse cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunocytochemistry - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Immunocytochemistry - Anti-EAAT1 antibody [EPR12686] (ab181036)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural / glia cells labelling EAAT1 with ab181036 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in rat primary glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

  • Immunocytochemistry - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Immunocytochemistry - Anti-EAAT1 antibody [EPR12686] (ab181036)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural / glia cells labelling EAAT1 with ab181036 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2 ug/ml dilution.

  • Immunocytochemistry - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Immunocytochemistry - Anti-EAAT1 antibody [EPR12686] (ab181036)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural / glia cells labelling EAAT1 with ab181036 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neural / glia cell. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

    ab181036 staining EAAT1 in rat cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

    ab181036 staining EAAT1 in human glioma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

    ab181036 staining EAAT1 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution + Human cerebellum at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 59 kDa
    Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.



    Blocking and diluting buffer: 5% NFDM/TBST

    The band above (100KDa) is dimer

  • Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)
    All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Human cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 59 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

    Immunohistochemical staining of EAAT1 in paraffin-embedded human brain tissue using ab181036 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-EAAT1 antibody [EPR12686] (ab181036)
    Anti-EAAT1 antibody [EPR12686] (ab181036)

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (6)

Publishing research using ab181036? Please let us know so that we can cite the reference in this datasheet.

ab181036 has been referenced in 6 publications.

  • Zheng J  et al. GLP-1 improves the supportive ability of astrocytes to neurons by promoting aerobic glycolysis in Alzheimer's disease. Mol Metab 47:101180 (2021). PubMed: 33556642
  • Zhang X  et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
  • Sun N  et al. DCX and CRABP2 are candidate genes for differential diagnosis between pre-chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients. Pediatr Investig 5:106-111 (2021). PubMed: 34179706
  • Sipe GO  et al. Astrocyte glutamate uptake coordinates experience-dependent, eye-specific refinement in developing visual cortex. Glia 69:1723-1735 (2021). PubMed: 33675674
  • Wang R  et al. Astrocytic JWA deletion exacerbates dopaminergic neurodegeneration by decreasing glutamate transporters in mice. Cell Death Dis 9:352 (2018). PubMed: 29500411
  • Liu J  et al. Nestin-expressing cell types in the temporal lobe and hippocampus: Morphology, differentiation, and proliferative capacity. Glia 66:62-77 (2018). IHC-P ; Human . PubMed: 28925561

Customer reviews and Q&As

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Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-EAAT1 [EPR12686] antibody

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Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citric acid 0.05% Tween20
Sample
Monkey Tissue sections (brain)
Specification
brain
Permeabilization
No
Fixative
Paraformaldehyde
Read More

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Submitted Feb 27 2014

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