Name: Anti-EAAT1 antibody [EPR12686]
Brand: RabMAb
SKU: ab181036
Rating: 5 (1 reviews)

Product name

Anti-EAAT1 antibody [EPR12686]
See all EAAT1 primary antibodies
Description

Rabbit monoclonal [EPR12686] to EAAT1
Host species

Rabbit
Tested applications

Suitable for: IHC-FoFr, IHC-P, WBmore details
Unsuitable for: ICC/IF
Species reactivity

Reacts with: Mouse, Rat, Human, Monkey
Immunogen

Synthetic peptide within Human EAAT1 aa 150-250. The exact sequence is proprietary.
Database link: P43003
Positive control
- Mouse brain, rat brain and human cerebellum lysates. Human brain tissue.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR12686
Isotype

IgG
Research areas

Alternative Versions
- Anti-EAAT1 antibody [EPR12686] - BSA and Azide free (ab240235)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Mouse brain tissue lysate - total protein (ab30151)
Related Products

Our Abpromise guarantee covers the use of ab181036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-FoFr		Use at an assay dependent concentration.
IHC-P		1/50 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB		1/1000 - 1/10000. Predicted molecular weight: 59 kDa.

Application notes

Is unsuitable for ICC/IF.

Function

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Tissue specificity

Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.
Involvement in disease

Defects in SLC1A3 are the cause of episodic ataxia type 6 (EA6) [MIM:612656]. EA6 is characterized by episodic ataxia, seizures, migraine and alternating hemiplegia.
Sequence similarities

Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A3 subfamily.
Post-translational
modifications

Glycosylated.
Cellular localization

Membrane.
Target information above from: UniProt accession P43003 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6507 Human
- Entrez Gene: 20512 Mouse
- Entrez Gene: 29483 Rat
- Omim: 600111 Human
- SwissProt: P43003 Human
- SwissProt: P56564 Mouse
- SwissProt: P24942 Rat
- Unigene: 481918 Human
- Unigene: 204834 Mouse
- Unigene: 34134 Rat
see all
Alternative names
- EA6 antibody
- EAA1_HUMAN antibody
- EAAT1 antibody
- Excitatory amino acid transporter 1 antibody
- FLJ25094 antibody
- GLAST antibody
- GLAST-1 antibody
- GLAST1 antibody
- Glial high affinity glutamate transporter antibody
- glutamate/aspartate transporter, high affinity, sodium-dependent antibody
- High affinity neuronal glutamate transporter antibody
- Slc1a3 antibody
- Sodium dependent glutamate/aspartate transporter antibody
- Sodium-dependent glutamate/aspartate transporter 1 antibody
- Solute carrier family 1 (glial high affinity glutamate transporter) member 3 antibody
- Solute carrier family 1 member 3 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in rat cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in mouse cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1: PBS in place of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in human glioma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1: PBS in place of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

ab181036 staining EAAT1 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/1000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1: PBS in place of primary antibody.
Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution

Lane 1 : Mouse brain
Lane 2 : Mouse hippocampus
Lane 3 : Rat hippocampus
Lane 4 : Rat brain

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 59 kDa
Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/5000 dilution + Human cerebellum at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 59 kDa
Additional bands at: 59 kDa. We are unsure as to the identity of these extra bands.

Blocking and diluting buffer: 5% NFDM/TBST

The band above (100KDa) is dimer
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)This image is courtesy of an anonymous Abreview

ab181036 staining EAAT1 in monkey brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, blocked with 10% serum at 24°C and antigen retrieval was by heat mediation in 10mM citrate acid + 0.05% Tween 20. The sample was incubated with primary antibody (1/100 in 2% donkey buffer) at 24°C for 13 hours. An Alexa Fluor® 647-conjugated donkey anti-rabbit polyclonal (1/100) was used as the secondary antibody.
See Abreview
Western blot - Anti-EAAT1 antibody [EPR12686] (ab181036)

All lanes : Anti-EAAT1 antibody [EPR12686] (ab181036) at 1/1000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Human cerebellum lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT1 antibody [EPR12686] (ab181036)

Immunohistochemical staining of EAAT1 in paraffin-embedded human brain tissue using ab181036 at a 1/50 dilution.

Immunohistochemistry protocols
Western blot protocols

This product has been referenced in:

Wang R et al. Astrocytic JWA deletion exacerbates dopaminergic neurodegeneration by decreasing glutamate transporters in mice. Cell Death Dis 9:352 (2018). Read more (PubMed: 29500411) »
Liu J et al. Nestin-expressing cell types in the temporal lobe and hippocampus: Morphology, differentiation, and proliferative capacity. Glia 66:62-77 (2018). IHC-P ; Human . Read more (PubMed: 28925561) »

See all 2 Publications for this product

Publishing research using ab181036? Please let us know so that we can cite the reference in this datasheet.

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Anti-EAAT1 antibody [EPR12686] (ab181036)

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Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-EAAT1 [EPR12686] antibody