Overview

  • Product name

  • Description

    Goat polyclonal to EAAT2
  • Host species

    Goat
  • Specificity

    Recommended for rat samples only.

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • ICC/IF: Rat pup brain heterogenous cells. WB: Rat brain lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 62 kDa.

Predicted MW: 60, 62 kDa (glycosylations and lipidations).

Dimerization expected.

ICC/IF Use a concentration of 15 µg/ml.

Target

  • Function

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • Sequence similarities

    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EAA2_HUMAN antibody
    • EAAT2 antibody
    • Excitatory amino acid transporter 2 antibody
    • Excitotoxic amino acid transporter 2 antibody
    • Glial high affinity glutamate transporter antibody
    • GLT 1 antibody
    • GLT1 antibody
    • Glutamate aspartate transporter II antibody
    • Glutamate transporter 1 antibody
    • Glutamate/aspartate transporter II antibody
    • Slc1a2 antibody
    • Sodium dependent glutamate aspartate transporter 2 antibody
    • Sodium-dependent glutamate/aspartate transporter 2 antibody
    • solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
    • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
    • Solute carrier family 1 member 2 antibody
    see all

Images

  • All lanes : Anti-EAAT2 antibody (ab235202) at 5 µg/ml

    Lane 1 : Rat brain lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat IgG [H&L] HRP at 1/40000 dilution

    Predicted band size: 62 kDa


    Exposure time: 60 seconds


    Predicted MW: 60, 62 kDa (glycosylations and lipidations).

    Dimerization expected.

  • 4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized rat pup brain heterogenous cells stained for EAAT2 (green) using ab235202 at 15 μg/ml (overnight at 4°C) in ICC/IF. Donkey Anti-Goat IgG DyLight™ 488 Preadsorbed was used as the secondary antibody 5 µg/ml (1 hour at RT). Nuclear counterstain was DAPI.

References

ab235202 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab235202.
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