• Product name
  • Description
    Rabbit polyclonal to EAAT2
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Rat EAAT2 aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P31596
    (Peptide available as ab41752)

  • Positive control
    • WB: Mouse and rat brain tissue lysates. IHC-Fr: Mouse brain tissue. ICC/IF: PC-12 cells.



Our Abpromise guarantee covers the use of ab41621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.


  • Function
    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • Sequence similarities
    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
  • Post-translational
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • EAA2_HUMAN antibody
    • EAAT2 antibody
    • Excitatory amino acid transporter 2 antibody
    • Excitotoxic amino acid transporter 2 antibody
    • Glial high affinity glutamate transporter antibody
    • GLT 1 antibody
    • GLT1 antibody
    • Glutamate aspartate transporter II antibody
    • Glutamate transporter 1 antibody
    • Glutamate/aspartate transporter II antibody
    • Slc1a2 antibody
    • Sodium dependent glutamate aspartate transporter 2 antibody
    • Sodium-dependent glutamate/aspartate transporter 2 antibody
    • solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
    • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
    • Solute carrier family 1 member 2 antibody
    see all


  • All lanes : Anti-EAAT2 antibody (ab41621) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Immunohistochemical staining of PFA-fixed, frozen mouse brain tissue using undiluted ab41621. Tissue sections were permeabilized using Triton-X100 and incubated with ab41621 for 12 hours at 4°C. ab150077 (goat anti-rabbit IgG H&L Alexa Fluor® 488) was used as the secondary antibody.

    See Abreview

  • Methanol fixed human astrocytes (differentiated from H9-derived neuronal) cells labeling EAAT2 using ab41621 at a 1/250 dilution, 1 hr, 22°C (green) followed by an Alexa Fluor® 488 secondary antibody (1/1000 dilution), in ICC/IF.

    Cells were blocked in 5% BSA for 30 mins, 22°C.

  • ICC/IF image of ab41621 stained PC-12 (rat adrenal gland pheochromocytoma cell line) cells.

    The cells were fixed in 4% formaldehyde (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41621, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.


This product has been referenced in:
  • Powell GL  et al. Chronic treatment with N-acetylcysteine decreases extinction responding and reduces cue-induced nicotine-seeking. Physiol Rep 7:e13958 (2019). Read more (PubMed: 30632301) »
  • Gomez JA  et al. Ventral tegmental area astrocytes orchestrate avoidance and approach behavior. Nat Commun 10:1455 (2019). Read more (PubMed: 30926783) »
See all 30 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (astrocytes differentiated from H9-derived neuronal)
astrocytes differentiated from H9-derived neuronal
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 30 2018

Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat Tissue sections (rat brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tri-sodium citrate buffer
Yes - triton 0.3%
rat brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: RT°C

Dr. Bartosz Pomierny

Verified customer

Submitted Mar 15 2018

Western blot
Cow Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 25 2017

Western blot
Mouse Tissue lysate - whole (Spinal cord)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Spinal cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 29 2016

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (Brain tissue)
Yes - Triton-X100
Brain tissue

Dr. Daniel Berg

Verified customer

Submitted Aug 20 2015


Thank you for contacting us.
I have contacted the Abreview customer regarding more information about the staining with ab41621. I will let you know if I hear back from her.
As for ab61859, I have the following IHC-P protocol:
General IHC Protocol
Paraffin Sections:
1. Deparaffinize sections in xylene, 2x5min.
2. Hydrate with 100% ethanol, 2x3min.
3. Hydrate with 95% ethanol, 1min.
4. Rinse in distilled water.
Tissue Staining:
1. Follow procedure for pretreatment as required.
2. Procedure for Immunoenzyme Staining.
3. Rinse Sections in Washing Buffer (PBS+0.1% Triton X-100, pH 7.4) for 2x2 min.
4. Serum Blocking: incubate sections in normal serum block – species same as secondary antibody( Wash Buffer + 5% Normal Sera). Note: since this protocol uses avidin-biotin detection system, avidin/biotin block may be needed based on tissue type. If you do, the avidin/biotin blocking should be done after normal serum block and before primary antibody incubation.
5. Primary Antibody: incubate sections in primary antibody at appropriate dilution in Wash Buffer for 1 hour at room temperature or overnight at 4C. Note:Do not rinse sections between serum block and primary antibody incubation.
6. Rinse in washing buffer for 3x2 min.
7. Peroxidase Blocking: incubate sections in peroxidase blocking solution for 10 minutes at room temperature.
8. Rinse in washing buffer for 3x2 min.
9. Secondary Antibody: incubate sections in biotinylated secondary antibody at an appropriate dilution in Wash Buffer for 30 minutes at room temperature.
10. Rinse in washing buffer for 3x2 min.
11. Detection: incubate sections in streptavidin-HRP in Wash Buffer for 30 minutes at room temperature.
12. Rinse in washing buffer for 3x2 min.
13. Chromagen/Substrate: incubate sections in peroxidase substrate solution.
14. Rinse in washing buffer for 3x2 min.
15. Rinse in running tap water for 2-5 minutes.
16. Dehydrate through 95% ethanol for 1 minute, 100% ethanol for 2x3min.
17. Clear in xylene for 2x5min.
18. Coverslip with mounting medium.

Peroxidase Block:
0.3% H2O2 in PBS (for Paraffin Sections)
0.3% H2O2 in Methanol (for Frozen Sections)

Avidin/Biotin Block
0.001% Avidin in PBS
0.001% Biotin in PBS
Block for 10 minutes with first solution, rinse with PBS, block for 10 minutes with second solution.
I am checking with the lab regarding the antigen retrieval method used with this antibody.
I hope this information is so far helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!

Read More
Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse Tissue sections (Brain sections)
Brain sections
Antigen retrieval step

Dr. Sophie Pezet

Verified customer

Submitted Jan 25 2010

For licensing inquiries, please contact partnerships@abcam.com

Sign up