Product nameAnti-EAAT2 antibody
See all EAAT2 primary antibodies
DescriptionRabbit polyclonal to EAAT2
Tested applicationsSuitable for: WB, ICC/IF, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human
- WB: Mouse and rat brain tissue lysates. IHC-Fr: Mouse brain tissue. ICC/IF: PC-12 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab41621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionTransports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Sequence similaritiesBelongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
- Information by UniProt
- EAA2_HUMAN antibody
- EAAT2 antibody
- Excitatory amino acid transporter 2 antibody
All lanes : Anti-EAAT2 antibody (ab41621) at 1 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Methanol fixed human astrocytes (differentiated from H9-derived neuronal) cells labeling EAAT2 using ab41621 at a 1/250 dilution, 1 hr, 22°C (green) followed by an Alexa Fluor® 488 secondary antibody (1/1000 dilution), in ICC/IF.
Cells were blocked in 5% BSA for 30 mins, 22°C.
ICC/IF image of ab41621 stained PC-12 (rat adrenal gland pheochromocytoma cell line) cells.
The cells were fixed in 4% formaldehyde (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41621, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
This product has been referenced in:
- Powell GL et al. Chronic treatment with N-acetylcysteine decreases extinction responding and reduces cue-induced nicotine-seeking. Physiol Rep 7:e13958 (2019). Read more (PubMed: 30632301) »
- Gomez JA et al. Ventral tegmental area astrocytes orchestrate avoidance and approach behavior. Nat Commun 10:1455 (2019). Read more (PubMed: 30926783) »