Overview

  • Product name
  • Description
    Rabbit polyclonal to EAAT2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Rat EAAT2 aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P31596
    (Peptide available as ab41752)

  • Positive control
    • WB: Mouse and rat brain tissue lysates. IHC-Fr: Mouse brain tissue. ICC/IF: PC-12 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab41621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • Sequence similarities
    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAA2_HUMAN antibody
    • EAAT2 antibody
    • Excitatory amino acid transporter 2 antibody
    • Excitotoxic amino acid transporter 2 antibody
    • Glial high affinity glutamate transporter antibody
    • GLT 1 antibody
    • GLT1 antibody
    • Glutamate aspartate transporter II antibody
    • Glutamate transporter 1 antibody
    • Glutamate/aspartate transporter II antibody
    • Slc1a2 antibody
    • Sodium dependent glutamate aspartate transporter 2 antibody
    • Sodium-dependent glutamate/aspartate transporter 2 antibody
    • solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
    • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
    • Solute carrier family 1 member 2 antibody
    see all

Images

  • All lanes : Anti-EAAT2 antibody (ab41621) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Immunohistochemical staining of PFA-fixed, frozen mouse brain tissue using undiluted ab41621. Tissue sections were permeabilized using Triton-X100 and incubated with ab41621 for 12 hours at 4°C. ab150077 (goat anti-rabbit IgG H&L Alexa Fluor® 488) was used as the secondary antibody.

    See Abreview

  • Methanol fixed human astrocytes (differentiated from H9-derived neuronal) cells labeling EAAT2 using ab41621 at a 1/250 dilution, 1 hr, 22°C (green) followed by an Alexa Fluor® 488 secondary antibody (1/1000 dilution), in ICC/IF.

    Cells were blocked in 5% BSA for 30 mins, 22°C.

  • ICC/IF image of ab41621 stained PC-12 (rat adrenal gland pheochromocytoma cell line) cells.

    The cells were fixed in 4% formaldehyde (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41621, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

References

This product has been referenced in:
  • Powell GL  et al. Chronic treatment with N-acetylcysteine decreases extinction responding and reduces cue-induced nicotine-seeking. Physiol Rep 7:e13958 (2019). Read more (PubMed: 30632301) »
  • Cicvaric A  et al. Enhanced synaptic plasticity and spatial memory in female but not male FLRT2-haplodeficient mice. Sci Rep 8:3703 (2018). Read more (PubMed: 29487336) »
See all 28 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-6 of 6 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (astrocytes differentiated from H9-derived neuronal)
Permeabilization
No
Specification
astrocytes differentiated from H9-derived neuronal
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Oct 30 2018

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (rat brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tri-sodium citrate buffer
Permeabilization
Yes - triton 0.3%
Specification
rat brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: RT°C
Fixative
Paraformaldehyde

Dr. Bartosz Pomierny

Verified customer

Submitted Mar 15 2018

Application
Western blot
Sample
Cow Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 25 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Spinal cord)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
Spinal cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 29 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain tissue)
Permeabilization
Yes - Triton-X100
Specification
Brain tissue
Fixative
Paraformaldehyde

Dr. Daniel Berg

Verified customer

Submitted Aug 20 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No

Dr. Sophie Pezet

Verified customer

Submitted Jan 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

Sign up