Overview

  • Product name

    Anti-EAAT2 antibody [EPR10769(2)]
    See all EAAT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10769(2)] to EAAT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EAAT2 aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: P43004

  • Positive control

    • SH-SY5Y, HeLa whole cell lysate (ab150035), Human fetal brain lysate, Rat liver lysates, Rat spleen and Mouse brain lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10769(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178401 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 62 kDa.

For unpurified use at 1/1000 - 1/10000.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
    • Sequence similarities

      Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
    • Post-translational
      modifications

      Glycosylated.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • EAA2_HUMAN antibody
      • EAAT2 antibody
      • Excitatory amino acid transporter 2 antibody
      • Excitotoxic amino acid transporter 2 antibody
      • Glial high affinity glutamate transporter antibody
      • GLT 1 antibody
      • GLT1 antibody
      • Glutamate aspartate transporter II antibody
      • Glutamate transporter 1 antibody
      • Glutamate/aspartate transporter II antibody
      • Slc1a2 antibody
      • Sodium dependent glutamate aspartate transporter 2 antibody
      • Sodium-dependent glutamate/aspartate transporter 2 antibody
      • solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
      • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
      • Solute carrier family 1 member 2 antibody
      see all

    Images

    • All lanes : Anti-EAAT2 antibody [EPR10769(2)] (ab178401) at 1/200 dilution (purified)

      Lane 1 : Mouse brain lysates
      Lane 2 : Rat spleen lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 62 kDa
      Observed band size: 65 kDa
      why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-EAAT2 antibody [EPR10769(2)] (ab178401) at 1/1000 dilution (purified)

      Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 3 : Rat liver lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 62 kDa
      Observed band size: 65 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-EAAT2 antibody [EPR10769(2)] (ab178401) at 1/1000 dilution (unpurified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 62 kDa

    • Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling EAAT2 with unpurified ab178401 at 1/10 dilution.

    References

    This product has been referenced in:

    • Shimamoto A  et al. Glutamate-Glutamine Transfer and Chronic Stress-Induced Sex Differences in Cocaine Responses. Neuroscience 391:104-119 (2018). Read more (PubMed: 30240589) »
    • Yao L  et al. Higher ambient synaptic glutamate at inhibitory versus excitatory neurons differentially impacts NMDA receptor activity. Nat Commun 9:4000 (2018). Read more (PubMed: 30275542) »
    See all 4 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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