Overview

  • Product name

    Anti-EAAT2 antibody [EPR19798]
    See all EAAT2 primary antibodies
  • Description

    Rabbit monoclonal [EPR19798] to EAAT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse EAAT2 aa 1-100. The exact sequence is proprietary.
    Database link: P43006

  • Positive control

    • WB: Mouse and rat brain lysates. IHC-P: Mouse and rat striatum tissues. IHC-Fr: Mouse striatum tissue. IP: Mouse brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19798
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
WB 1/1000. Detects a band of approximately 65,180 kDa (predicted molecular weight: 62 kDa).
IP 1/30.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
  • Sequence similarities

    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A2 subfamily.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EAA2_HUMAN antibody
    • EAAT2 antibody
    • Excitatory amino acid transporter 2 antibody
    • Excitotoxic amino acid transporter 2 antibody
    • Glial high affinity glutamate transporter antibody
    • GLT 1 antibody
    • GLT1 antibody
    • Glutamate aspartate transporter II antibody
    • Glutamate transporter 1 antibody
    • Glutamate/aspartate transporter II antibody
    • Slc1a2 antibody
    • Sodium dependent glutamate aspartate transporter 2 antibody
    • Sodium-dependent glutamate/aspartate transporter 2 antibody
    • solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody
    • Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody
    • Solute carrier family 1 member 2 antibody
    see all

Images

  • All lanes : Anti-EAAT2 antibody [EPR19798] (ab205248) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat liver lysate
    Lane 6 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
    Lane 7 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 8 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 10 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 62 kDa
    Observed band size: 180,65 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    1. EAAT2 is a multi-pass membrane protein, the main transporter that clears the excitatory neurotransmitter glutamate in the central nervous system. The bands around 180 kDa are multimers of EAAT2, which is consistent with what have been described in the literatures (PMID: 24569372 & 20193040).

    2. EATT2 is primarily expressed in astrocytes but is also expressed in neurons of the retina and during fetal development(PMID:12176072).

  • Immunohistochemical analysis of paraffin-embedded mouse striatum tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on mouse striatum is observed [PMID 25391854]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on mouse kidney [PMID: 11038258].

    Counter stained with Hematoxylin.

     

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat striatum tissue labeling EAAT2 with ab205248 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Rat striatum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse striatum tissue labeling EAAT2 with ab205248 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membrane staining on mouse striatum is observed [PMID: 25391854].  The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077  at 1/1000 dilution.

  • EAAT2 was immunoprecipitated from 0.35 mg of Mouse brain lysate with ab205248 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205248 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse brain lysate, 10μg (Input).

    Lane 2: ab205248 IP in Mouse brain lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]- Isotype Control (ab172730) instead of ab205248 in Mouse brain lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

This product has been referenced in:

  • Servaes S  et al. Neuroreceptor kinetics in rats repeatedly exposed to quinpirole as a model for OCD. PLoS One 14:e0213313 (2019). Read more (PubMed: 30845202) »
See 1 Publication for this product

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