Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
- Datasheet
- Specific References (5)
- Protocols
Overview
-
Product nameAnti-EAAT3 antibody [EPR6774(B)]
See all EAAT3 primary antibodies -
DescriptionRabbit monoclonal [EPR6774(B)] to EAAT3
-
Host speciesRabbit
-
Tested applicationsSuitable for: Flow Cyt, IHC-P, ICC/IF, WBmore details
Unsuitable for: IP -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human EAAT3 aa 150-250. The exact sequence is proprietary.
-
Positive control
- WB: Human fetal brain, human testis, mouse brain and rat brain tissue lysates. IHC-P: Human kidney tissue. Flow Cyt: U-87 MG cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Dissociation constant (KD)KD = 3.80 x 10 -12 M Learn more about KD
-
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR6774(B)
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab124802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/200. | |
| IHC-P | 1/500 - 1/1000. | |
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
Target
-
FunctionTransports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5.
-
Tissue specificityExpressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
-
Sequence similaritiesBelongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily.
-
Post-translational
modificationsGlycosylated. -
Cellular localizationMembrane.
- Information by UniProt
-
Database links
- Entrez Gene: 6505 Human
- Entrez Gene: 20510 Mouse
- Entrez Gene: 25550 Rat
- Omim: 133550 Human
- SwissProt: P43005 Human
- SwissProt: P51906 Mouse
- SwissProt: P51907 Rat
- Unigene: 444915 Human
see all -
Alternative names
- EAA3_HUMAN antibody
- EAAC 1 antibody
- EAAC1 antibody
see all
Images
-
![Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)](https://www.abcam.com/ps/products/124/ab124802/Images/ab124802-266420-ab124802WB1.jpg)
Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)All lanes : Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/50000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Observed band size: 70 kDa (why is the actual band size different from the predicted?)
Exposure time: 30 secondsBlocking and Diluting buffer : 5% NFDM/TBST
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)](https://www.abcam.com/ps/products/124/ab124802/Images/EAAT3-Primary-antibodies-ab124802-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)ab124802, at 1/500 dilution, staining EAAT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry. -
![Flow Cytometry - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)](https://www.abcam.com/ps/products/124/ab124802/Images/ab124802-287296-ab124802FC1.jpg)
Flow Cytometry - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)Flow cytometry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling EAAT3 (red) with ab124802 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
-
![Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)](https://www.abcam.com/ps/products/124/ab124802/Images/ab124802-266422-ab124802WB2.jpg)
Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)All lanes : Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/10000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Observed band size: 70 kDa (why is the actual band size different from the predicted?)
Exposure time: 1 minuteBlocking and Diluting buffer : 5% NFDM/TBST
An additional band detected at 100 kDa is unidentified.
-
![Other - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)](https://www.abcam.com/ps/products/124/ab124802/Images/EAAT3-Primary-antibodies-ab124802-3.jpg)
Other - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
Protocols
References
This product has been referenced in:
- González LF et al. Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice. Biol Res 50:29 (2017). Read more (PubMed: 28927446) »
- Huang C et al. Estrogen regulates excitatory amino acid carrier 1 (EAAC1) expression through sphingosine kinase 1 (SphK1) transacting FGFR-mediated ERK signaling in rat C6 astroglial cells. Neuroscience 319:9-22 (2016). Read more (PubMed: 26804240) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"