Recombinant Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
![Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)](https://www.abcam.com/ps/products/124/ab124802/Images/ab124802-266420-anti-eaat3-antibody-epr6774b-western-blot.jpg "Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6774(B)] to EAAT3
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-EAAT3 antibody [EPR6774(B)]
See all EAAT3 primary antibodies -
Description
Rabbit monoclonal [EPR6774(B)] to EAAT3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human EAAT3 aa 150-250. The exact sequence is proprietary.
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Positive control
- WB: Human fetal brain, human brain and human testis lysates . IHC-P: Human kidney tissue, and human glioma tissue. Flow Cyt: U-87 MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.80 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6774(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB | (1) |
1/10000 - 1/50000.
Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
| Notes |
|---|
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
Target
-
Function
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5. -
Tissue specificity
Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers). -
Sequence similarities
Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6505 Human
- Omim: 133550 Human
- SwissProt: P43005 Human
- Unigene: 444915 Human
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Alternative names
- EAA3_HUMAN antibody
- EAAC 1 antibody
- EAAC1 antibody
see all
Images
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Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)All lanes : Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/50000 dilution ((unpurified))
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and Diluting buffer : 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)](https://www.abcam.com/ps/products/124/ab124802/Images/ab124802-7-anti-eaat3-antibody-epr6774-b-immunohistochemistry-glioma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling EAAT3 with purified ab124802 at 1/1000 dilution (0.8 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)](https://www.abcam.com/ps/products/124/ab124802/Images/ab124802-365448-anti-eaat3-antibody-epr6774-b-westernblot-brain-human.jpg)
Western blot - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)Anti-EAAT3 antibody [EPR6774(B)] (ab124802) at 1/1000 dilution (Purified) + Human brain lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 70 kDa why is the actual band size different from the predicted?We are unsure how to define the extra bands.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)](https://www.abcam.com/ps/products/124/ab124802/Images/ab124802-138701-anti-eaat3-antibody-epr6774b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)Unpurified ab124802, at 1/500 dilution, staining EAAT3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-EAAT3 antibody [EPR6774(B)] (ab124802)](https://www.abcam.com/ps/products/124/ab124802/Images/ab124802-138699-anti-eaat3-antibody-epr6774b.jpg)
OI-RD Scanning - Anti-EAAT3 antibody [EPR6774(B)] (ab124802) -
![Anti-EAAT3 antibody [EPR6774(B)] (ab124802)](https://www.abcam.com/ps/products/124/ab124802/Images/ab124802-9-benefits-of-recombinant-antibodies.png)
Anti-EAAT3 antibody [EPR6774(B)] (ab124802)
Protocols
Datasheets and documents
References (9)
ab124802 has been referenced in 9 publications.
- Liu XX et al. Endothelial Cdk5 deficit leads to the development of spontaneous epilepsy through CXCL1/CXCR2-mediated reactive astrogliosis. J Exp Med 217:N/A (2020). PubMed: 31699822
- Hövel FFV et al. FGF-2 isoforms influence the development of dopaminergic neurons in the murine substantia nigra, but not anxiety-like behavior, stress susceptibility, or locomotor behavior. Behav Brain Res 374:112113 (2019). PubMed: 31381976
- Biolley C et al. Punicalagin increases glutamate absorption in differentiated Caco-2 cells by a mechanism involving gene expression regulation of an EAAT3 transporter. Food Funct 10:5333-5338 (2019). PubMed: 31389458
- Garza-Lombó C et al. Systemic L-buthionine-S-R-sulfoximine administration modulates glutathione homeostasis via NGF/TrkA and mTOR signaling in the cerebellum. Neurochem Int 121:8-18 (2018). PubMed: 30300680
- González LF et al. Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice. Biol Res 50:29 (2017). PubMed: 28927446
- Huang C et al. Estrogen regulates excitatory amino acid carrier 1 (EAAC1) expression through sphingosine kinase 1 (SphK1) transacting FGFR-mediated ERK signaling in rat C6 astroglial cells. Neuroscience 319:9-22 (2016). PubMed: 26804240
- Beaudin S & Welsh J 1,25-Dihydroxyvitamin D induces the glutamate transporter SLC1A1 and alters glutamate handling in non-transformed mammary cells. Mol Cell Endocrinol 424:34-41 (2016). WB ; Human . PubMed: 26774511
- Ramos-Chávez LA et al. Neurological effects of inorganic arsenic exposure: altered cysteine/glutamate transport, NMDA expression and spatial memory impairment. Front Cell Neurosci 9:21 (2015). WB ; Mouse . PubMed: 25709567
- Martinez-Lozada Z et al. Activation of sodium-dependent glutamate transporters regulates the morphological aspects of oligodendrocyte maturation via signaling through calcium/calmodulin-dependent kinase IIß's actin-binding/-stabilizing domain. Glia 62:1543-58 (2014). PubMed: 24866099
