Recombinant
RabMAb

Recombinant Anti-EAAT3 antibody [EPR6774(B)] (HRP) (ab203479)

Overview

  • Product name

    Anti-EAAT3 antibody [EPR6774(B)] (HRP)
    See all EAAT3 primary antibodies
  • Description

    Rabbit monoclonal [EPR6774(B)] to EAAT3 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human EAAT3 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • WB: Fetal brain, Human testis and Fetal muscle tissue lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab203479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. Negatively regulated by ARL6IP5.
  • Tissue specificity

    Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers).
  • Sequence similarities

    Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A1 subfamily.
  • Post-translational
    modifications

    Glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EAA3_HUMAN antibody
    • EAAC 1 antibody
    • EAAC1 antibody
    • EAAT 3 antibody
    • Excitatory amino acid carrier 1 antibody
    • Excitatory amino acid carrier1 antibody
    • Excitatory amino acid transporter 3 antibody
    • Excitatory amino acid transporter3 antibody
    • Excitatory amino-acid carrier 1 antibody
    • GLUTAMATE TRANSPORTER, HIGH-AFFINITY antibody
    • MEAAC 1 antibody
    • MEAAC1 antibody
    • Neuronal and epithelial glutamate transporter antibody
    • REAAC 1 antibody
    • REAAC1 antibody
    • Slc1 a1 antibody
    • Slc1a 1 antibody
    • SLC1A1 antibody
    • Sodium dependent glutamate/aspartate transporter 3 antibody
    • Sodium-dependent glutamate/aspartate transporter 3 antibody
    • Solute carrier family 1 (neuronal / epithelial high affinity glutamate transporter, system Xag), member 1 antibody
    • SOLUTE CARRIER FAMILY 1 (NEURONAL/EPITHELIAL HIGH AFFINITY GLUTAMATE TRANSPORTER), MEMBER 1 antibody
    • Solute carrier family 1 (neuronal/epithelial high affinity glutamate transporter, system Xag), member 1 antibody
    • Solute carrier family 1 member 1 antibody
    • Solute carrier family 1, member 1 antibody
    see all

Images

  • All lanes : Anti-EAAT3 antibody [EPR6774(B)] (HRP) (ab203479) at 1/5000 dilution

    Lane 1 : Brain (Human) Tissue Lysate - fetal normal tissue
    Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue
    Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab203479 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab203479 has not yet been referenced specifically in any publications.

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