Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/200
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
This Ab is positive for mouse FFPW sections,in what seem to me to be accepted localisation for EAAT4. The positivity is not as distinct as rat but, I think that this is due to my mouse cerebellum preparation/orientation. However, the pattern of positivity on mouse, rat and marmoset appears to me to be very similar: the only variation appears to be in the Glomeruli of the Granule cell layer.
Abcam response
Many thanks for your feedback on ab41650 (EAAT4 antibody) in IHC-P mouse cerebellum sections.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
Submitted Apr 20 2010