Anti-EAAT5 antibody (ab230217)

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Overview

  • Product name

    Anti-EAAT5 antibody
    See all EAAT5 primary antibodies

  • Description

    Rabbit polyclonal to EAAT5

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Recombinant fragment corresponding to Human EAAT5 aa 115-220.
    Sequence:

    HPGSAAQKETTEQSGKPIMSSADALLDLIRNMFPANLVEATFKQYRTKTT PVVKSPKVAPEEAPPRRILIYGVQEENGSHVQNFALDLTPPPEVVYKSEP GTSDGM


    Database link: O00341

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat heart tissue lysate. IHC-P: Human kidney tissue. ICC/IF: HepG2 cells.

  • General notes

     This product was previously labelled as SLC1A7

     

Properties

Applications

Our Abpromise guarantee covers the use of ab230217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61,17 kDa).

Target

  • Function

    Transports L-glutamate; the L-glutamate uptake is sodium- and voltage-dependent and chloride-independent. Its associated chloride conductance may participate in visual processing.

  • Tissue specificity

    Expressed primarily in retina. Detectable in liver, heart, muscle and brain.

  • Sequence similarities

    Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A7 subfamily.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • AAAT antibody
      • EAA5_HUMAN antibody
      • EAAT5 antibody
      • Excitatory amino acid transporter 5 (retinal glutamate transporter) antibody
      • Excitatory amino acid transporter 5 antibody
      • FLJ36602 antibody
      • Retinal glutamate transporter antibody
      • Slc1a7 antibody
      • Solute carrier family 1 (glutamate transporter) member 7 antibody
      • Solute carrier family 1 member 7 antibody
      see all

    Images

    • Western blot - Anti-EAAT5 antibody (ab230217)
      Western blot - Anti-EAAT5 antibody (ab230217)
      Anti-EAAT5 antibody (ab230217) at 1/500 dilution + Rat heart tissue lysate

      Secondary
      Goat anti-rabbit IgG at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 61,17 kDa
      Observed band size: 61 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT5 antibody (ab230217)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EAAT5 antibody (ab230217)

      Paraffin-embedded human kidney tissue stained for EAAT5 using ab230217 at 1/100 dilution in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-EAAT5 antibody (ab230217)
      Immunocytochemistry/ Immunofluorescence - Anti-EAAT5 antibody (ab230217)

      HepG2 (human liver hepatocellular carcinoma cell line) cells stained for EAAT5 using ab230217 at 1/100 dilution in ICC/IF. Secondary used is an Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

    References

    ab230217 has not yet been referenced specifically in any publications.

    Publishing research using ab230217? Please let us know so that we can cite the reference in this datasheet.

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