Overview

  • Product name

  • Description

    Rabbit polyclonal to EAF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human EAF2 aa 6-100.
    Database link: Q96CJ1

  • Positive control

    • WB: Mouse kidney tissue lysate. IHC-P: Human appendix tissue. ICC/IF: HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab237753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 29 kDa.
IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/500.

Target

  • Function

    Acts as a transcriptional transactivator of TCEA1 elongation activity (By similarity). Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.
  • Sequence similarities

    Belongs to the EAF family.
  • Cellular localization

    Nucleus speckle. Colocalizes with ELL to nuclear speckles.
  • Information by UniProt
  • Database links

  • Alternative names

    • BM 040 antibody
    • BM040 antibody
    • EAF 2 antibody
    • EAF2 antibody
    • EAF2_HUMAN antibody
    • Ehrlich S II transcriptional activator factor antibody
    • ELL associated factor 2 antibody
    • ELL-associated factor 2 antibody
    • Festa antibody
    • Testosterone regulated apoptosis inducer and tumor suppressor antibody
    • Testosterone regulated apoptosis inducer and tumor suppressor protein antibody
    • Testosterone-regulated apoptosis inducer and tumor suppressor protein antibody
    • TRAITS antibody
    • U19 antibody
    • Uncharacterized bone marrow protein BM 040 antibody
    • Uncharacterized bone marrow protein BM040 antibody
    see all

Images

  • Anti-EAF2 antibody (ab237753) at 1/500 dilution + Mouse kidney tissue lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 29 kDa

  • Paraffin-embedded human appendix tissue stained for EAF2 using ab237753 at 1/1,000 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

  • HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for EAF2 (green) using ab237753 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor® 488 conjugated Goat Anti-Rabbit IgG (H+L).

References

ab237753 has not yet been referenced specifically in any publications.

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