Product nameAnti-EAG2 antibody
See all EAG2 primary antibodies
DescriptionRabbit polyclonal to EAG2
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- HepG2 cell lysate and human brain tissue.
Previously labelled as KCNH5.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab32975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 112 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
RelevanceVoltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. The EAG2 gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. EAG2 is not expressed in differentiating myoblasts.
Cellular localizationCell Membrane: multi-pass membrane protein.
- EAG2 antibody
- Ether a go go potassium channel 2 antibody
- H EAG2 antibody
Anti-EAG2 antibody (ab32975) at 0.5 µg/ml + HepG2 cell lysate
HRP-conjugated anti-rabbit IgG at 1/50000 dilution
Predicted band size: 112 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling EAG2 at 4-8µg/ml. Neural cells with positive label are indicated by arrows.
This product has been referenced in:
- Huang X et al. Voltage-gated potassium channel EAG2 controls mitotic entry and tumor growth in medulloblastoma via regulating cell volume dynamics. Genes Dev 26:1780-96 (2012). Read more (PubMed: 22855790) »
- Macaulay EC et al. Hypomethylation of functional retrotransposon-derived genes in the human placenta. Mamm Genome 22:722-35 (2011). IHC-P ; Human . Read more (PubMed: 21874386) »