Anti-Eagle antibody [8B11DH10] (ab201327)

Overview

  • Product name

    Anti-Eagle antibody [8B11DH10]
  • Description

    Mouse monoclonal [8B11DH10] to Eagle
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, IHC (PFA fixed)more details
  • Species reactivity

    Reacts with: Drosophila melanogaster
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • Drosophila stage 15 embryos.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...
  • Purification notes

    Purity: 95%
  • Clonality

    Monoclonal
  • Clone number

    8B11DH10
  • Isotype

    IgG1
  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab201327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC (PFA fixed) Use at an assay dependent concentration.

Target

  • Relevance

    May play a critical role in development of the progeny of eagle-positive neuroblasts.
  • Cellular localization

    Nucleus
  • Database links

    • Alternative names

      • CG7383 antibody
      • eg antibody
      • egon antibody
      • NR0A3 antibody
      • Nuclear receptor subfamily 0 group A member 3 antibody
      • Protein eagle antibody
      • Protein embryonic gonad antibody
      • Protein spready antibody
      • spry antibody
      see all

    Images

    • Immunohistochemistry  analysis in neuroblast nuclei from Drosophila stage 15 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum.The Eagle antibody (ab201327) was used at 3.8 µg/mL, overnight at 4°C. The secondary antibody used was Alexa488 conjugated goat anti-mouse at 1/400.

    References

    ab201327 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab201327.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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