Key features and details
- Mouse monoclonal [8B11DH10] to Eagle
- Suitable for: ICC/IF, IHC (PFA fixed)
- Reacts with: Drosophila melanogaster
- Isotype: IgG1
Product nameAnti-Eagle antibody [8B11DH10]
DescriptionMouse monoclonal [8B11DH10] to Eagle
Tested applicationsSuitable for: ICC/IF, IHC (PFA fixed)more details
Species reactivityReacts with: Drosophila melanogaster
The details of the immunogen for this antibody are not available.
- Drosophila stage 15 embryos.
This antibody clone is manufactured by Abcam.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline
Concentration information loading...
Purification notesPurity: 95%
Light chain typekappa
Our Abpromise guarantee covers the use of ab201327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC (PFA fixed)||Use at an assay dependent concentration.|
RelevanceMay play a critical role in development of the progeny of eagle-positive neuroblasts.
- CG7383 antibody
- eg antibody
- egon antibody
Immunohistochemistry analysis in neuroblast nuclei from Drosophila stage 15 embryos. The cells were fixed in 4% formaldehyde for 25 minutes, permeabilized with 0.1% Triton X-100/PBS, and blocked in 0.1% Triton X-100/PBS, 2.5% Normal Goat Serum, 2.5% Normal Donkey Serum.The Eagle antibody (ab201327) was used at 3.8 µg/mL, overnight at 4°C. The secondary antibody used was Alexa488 conjugated goat anti-mouse at 1/400.
ab201327 has not yet been referenced specifically in any publications.