Key features and details
- Rabbit polyclonal to EAN57
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EAN57 antibody
DescriptionRabbit polyclonal to EAN57
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human EAN57 aa 101-233.
Database link: O43247
- IHC-P: Human testis tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121241 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use buffer at pH 6 for antigen retrieval.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
- Information by UniProt
- C22orf33 antibody
- cE81G9.2 antibody
- Chromosome 22 open reading frame 33 antibody
Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) of Human testis tissue sections labelling EAN57 with ab121241 (1/20). Antigen retrieval was by HIER pH6. Immunohistochemical staining shows strong cytoplasmic and nuclear positivity in a subset of cells in seminiferus ducts.
All lanes : Anti-EAN57 antibody (ab121241) at 1/250 dilution
Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2 : Negative control (vector only transfected HEK293T lysate)
Lane 3 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
ab121241 has not yet been referenced specifically in any publications.