• Product name
  • Description
    Rabbit polyclonal to EAP30
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human EAP30 aa 1-50 (N terminal). The exact sequence is proprietary.


    Database link: Q96H20


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab22768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 29 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.


  • Function
    Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4.
  • Sequence similarities
    Belongs to the SNF8 family.
  • Cellular localization
    Cytoplasm. Endosome membrane. Nucleus. Late endosome membrane. Recruited to the endosome membrane to participate in vesicle formation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dot3 antibody
    • ELL associated protein of 30 kDa antibody
    • ELL-associated protein of 30 kDa antibody
    • ESCRT-II complex subunit VPS22 antibody
    • hVps22 antibody
    • snf8 antibody
    • SNF8 ESCRTII complex subunit homolog (S cerevisiae) antibody
    • SNF8_HUMAN antibody
    • Vacuolar sorting protein SNF8 antibody
    • Vacuolar-sorting protein SNF8 antibody
    • VPS22 antibody
    see all


  • Anti-EAP30 antibody (ab22768) at 5 µg/ml + HepG2 Cell Lysate

    HRP conjugated anti-Rabbit IgG diluted in 1/50,000 - 100,000

    Performed under reducing conditions.

    Observed band size: 29 kDa
    why is the actual band size different from the predicted?

  • ab22768, at a 4.0-8.0 ug/ml dilution, staining human EAP30 in human stomach by immunohistochemistry, paraffin embedded tissue. Cells with positive label: epithelial cells of fundic gland (indicated with arrows) and surface mucous cells (indicated with arrow heads)
  • ab22768 staining EAP30 in Human heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).
  • ab22768 staining EAP30 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).


ab22768 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. You have followed the protocol recommended by our datasheet including the dilutions and blocking agent. Furthermore Eap30 expression has been shown in HeLa cells in the following publication: Martin-Serrano J, Yarovoy A, Perez-Caballero D, Bieniasz PD. Divergent retroviral late-budding domains recruit vacuolar protein sorting factors by using alternative adaptor proteins. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12414-9. Epub 2003 Sep 30. PMID: 14519844 However, my concern is that the detection of Eap30 has to my record only been shown at the transcript level by PCR amplification and therefore the level of protein expression may be insufficient for western immunoblot detection. I would therefore like to recommend that you incorporate a positive control in your experiments. I would like to recommend that the human hepatoma cell line HepG2 are used as a positive control; as shown in the western blotting image on our datasheet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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