Overview

  • Product name

  • Description

    Rabbit polyclonal to EAP30
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human EAP30 (internal sequence). The exact sequence is proprietary.
    Database link: Q96H20

  • Positive control

    • IHC-P: Human colon carcinoma tissue. WB: HeLa whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.

Target

  • Function

    Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4.
  • Sequence similarities

    Belongs to the SNF8 family.
  • Cellular localization

    Cytoplasm. Endosome membrane. Nucleus. Late endosome membrane. Recruited to the endosome membrane to participate in vesicle formation.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dot3 antibody
    • ELL associated protein of 30 kDa antibody
    • ELL-associated protein of 30 kDa antibody
    • ESCRT-II complex subunit VPS22 antibody
    • hVps22 antibody
    • snf8 antibody
    • SNF8 ESCRTII complex subunit homolog (S cerevisiae) antibody
    • SNF8_HUMAN antibody
    • Vacuolar sorting protein SNF8 antibody
    • Vacuolar-sorting protein SNF8 antibody
    • VPS22 antibody
    see all

Images

  • Paraffin-embedded human colon carcinoma tissue stained for EAP30 using ab228915 at 1/250 dilution in immunohistochemical analysis.

  • Anti-EAP30 antibody (ab228915) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Predicted band size: 29 kDa



    12% SDS-PAGE gel.

References

ab228915 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228915.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up