Anti-EB2 antibody (ab234843)
Key features and details
- Rabbit polyclonal to EB2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-EB2 antibody
See all EB2 primary antibodies -
Description
Rabbit polyclonal to EB2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human EB2 aa 159-240.
Database link: Q15555 -
Positive control
- WB: Mouse brain tissue lysate. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Predicted molecular weight: 37 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 37 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
Tissue specificity
Expressed in different tumor cell lines. Up-regulated in activated B- and T-lymphocytes. -
Sequence similarities
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
Domain
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
Cellular localization
Cytoplasm > cytoskeleton. Associated with the microtubule network. Accumulates at the plus end of microtubules. - Information by UniProt
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Database links
- Entrez Gene: 421105 Chicken
- Entrez Gene: 510159 Cow
- Entrez Gene: 10982 Human
- Entrez Gene: 212307 Mouse
- Entrez Gene: 679221 Rat
- Omim: 605789 Human
- SwissProt: Q5ZKK1 Chicken
- SwissProt: 3SZP2 Cow
see all -
Alternative names
- APC binding protein EB1 antibody
- APC binding protein EB2 antibody
- APC-binding protein EB2 antibody
see all
Images
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HepG2 (human liver hepatocellular carcinoma cell line) cells labeling EB2 (green) using ab234843 at 1/100 dilution in ICC/IF. Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody.
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Anti-EB2 antibody (ab234843) at 1/500 dilution + Mouse brain tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab234843 has not yet been referenced specifically in any publications.