Key features and details
- Rabbit polyclonal to EB2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EB2 antibody
See all EB2 primary antibodies
DescriptionRabbit polyclonal to EB2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Orangutan
- IHC-P: Human cerebral cortex and colon tissue. WB: MAPRE2 overexpression HEK-293T lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab253007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
Tissue specificityExpressed in different tumor cell lines. Up-regulated in activated B- and T-lymphocytes.
Sequence similaritiesBelongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain.
DomainComposed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
Cellular localizationCytoplasm > cytoskeleton. Associated with the microtubule network. Accumulates at the plus end of microtubules.
- Information by UniProt
- APC binding protein EB1 antibody
- APC binding protein EB2 antibody
- APC-binding protein EB2 antibody
Paraffin-embedded human cerebral-cortex tissue stained for EB2 using ab253007 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-EB2 antibody (ab253007) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : MAPRE2 overexpression HEK-293T lysate
Paraffin-embedded human colon tissue stained for EB2 using ab253007 at 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for EB2 using ab253007 at 1/50 dilution in immunohistochemical analysis. No positivity in hepatocytes as expected.
Paraffin-embedded human testis tissue stained for EB2 using ab253007 at 1/50 dilution in immunohistochemical analysis. No positivity in cells in seminiferous ducts as expected.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab253007 has not yet been referenced specifically in any publications.