Anti-EB2 antibody [KT52] (ab45767)
Key features and details
- Rat monoclonal [KT52] to EB2
- Suitable for: ICC/IF
- Reacts with: Recombinant fragment
- Isotype: IgG2b
Overview
-
Product name
Anti-EB2 antibody [KT52]
See all EB2 primary antibodies -
Description
Rat monoclonal [KT52] to EB2 -
Host species
Rat -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant full length EB2 protein (Human)
-
Positive control
- CHO cell lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
KT52 -
Isotype
IgG2b -
Research areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
Notes |
---|
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
Tissue specificity
Expressed in different tumor cell lines. Up-regulated in activated B- and T-lymphocytes. -
Sequence similarities
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
Domain
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
Cellular localization
Cytoplasm > cytoskeleton. Associated with the microtubule network. Accumulates at the plus end of microtubules. - Information by UniProt
-
Alternative names
- APC binding protein EB1 antibody
- APC binding protein EB2 antibody
- APC-binding protein EB2 antibody
see all
Images
Protocols
Datasheets and documents
-
Datasheet download
References (6)
ab45767 has been referenced in 6 publications.
- Van Bergen NJ et al. Abnormalities of mitochondrial dynamics and bioenergetics in neuronal cells from CDKL5 deficiency disorder. Neurobiol Dis N/A:105370 (2021). PubMed: 33905871
- Terzic B et al. Temporal manipulation of Cdkl5 reveals essential postdevelopmental functions and reversible CDKL5 deficiency disorder-related deficits. J Clin Invest 131:N/A (2021). PubMed: 34651584
- Gong L et al. WDHD1 Leads to Cisplatin Resistance by Promoting MAPRE2 Ubiquitination in Lung Adenocarcinoma. Front Oncol 10:461 (2020). PubMed: 32426268
- Tang S et al. Altered NMDAR signaling underlies autistic-like features in mouse models of CDKL5 deficiency disorder. Nat Commun 10:2655 (2019). PubMed: 31201320
- Baltussen LL et al. Chemical genetic identification of CDKL5 substrates reveals its role in neuronal microtubule dynamics. EMBO J 37:N/A (2018). PubMed: 30266824
- Asanov A et al. A relay mechanism between EB1 and APC facilitate STIM1 puncta assembly at endoplasmic reticulum-plasma membrane junctions. Cell Calcium 54:246-56 (2013). PubMed: 23871111