Overview

  • Product name

  • Description

    Rabbit polyclonal to EB3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 66-264 of Human EB3 (Q9UPY8).

  • Positive control

    • MCF7 and Mouse brain whole cell lysates; Human lung carcinoma tissue.
  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab126953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • Tissue specificity

    Predominantly expressed in brain and muscle.
  • Sequence similarities

    Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • Domain

    Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • Cellular localization

    Cytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt
  • Database links

  • Alternative names

    • APC binding protein antibody
    • EB 3 antibody
    • EB1 protein family member 3 antibody
    • EB3 antibody
    • EBF 3 antibody
    • EBF-3 antibody
    • EBF3 antibody
    • EBF3 S antibody
    • End binding protein 3 antibody
    • End-binding protein 3 antibody
    • MAPRE 3 antibody
    • MAPRE3 antibody
    • MARE3_HUMAN antibody
    • Member 3 antibody
    • Microtubule Associated Protein antibody
    • Microtubule associated protein RP/EB family member 3 antibody
    • Microtubule-associated protein RP/EB family member 3 antibody
    • OTTHUMP00000200910 antibody
    • RP 3 antibody
    • RP/EB Family antibody
    • RP3 antibody
    see all

Images

  • ab126953, at 1/500 dilution, staining EB3 in paraffin embedded Human lung carcinoma tissue by Immunohistochemistry.
  • Anti-EB3 antibody (ab126953) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 31 kDa



    12% SDS PAGE
  • Anti-EB3 antibody (ab126953) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 31 kDa



    12% SDS PAGE

References

This product has been referenced in:

  • Nakamura S  et al. Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells. PLoS One 7:e51442 (2012). WB ; Human . Read more (PubMed: 23251535) »
See 1 Publication for this product

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