Overview

  • Product name

  • Description

    Goat polyclonal to EB3
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human EB3 aa 151-164 (internal sequence) (Cysteine residue).
    Sequence:

    C-KLIGTAVPQRTSPT


    Database link: Q9UPY8

  • Positive control

    • WB: Human brain lysate; rat brain lysate; mouse brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab223665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 32 kDa).

Target

  • Function

    May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • Tissue specificity

    Predominantly expressed in brain and muscle.
  • Sequence similarities

    Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • Domain

    Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • Cellular localization

    Cytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt
  • Database links

  • Alternative names

    • APC binding protein antibody
    • EB 3 antibody
    • EB1 protein family member 3 antibody
    • EB3 antibody
    • EBF 3 antibody
    • EBF-3 antibody
    • EBF3 antibody
    • EBF3 S antibody
    • End binding protein 3 antibody
    • End-binding protein 3 antibody
    • MAPRE 3 antibody
    • MAPRE3 antibody
    • MARE3_HUMAN antibody
    • Member 3 antibody
    • Microtubule Associated Protein antibody
    • Microtubule associated protein RP/EB family member 3 antibody
    • Microtubule-associated protein RP/EB family member 3 antibody
    • OTTHUMP00000200910 antibody
    • RP 3 antibody
    • RP/EB Family antibody
    • RP3 antibody
    see all

Images

  • All lanes : Anti-EB3 antibody (ab223665) at 0.1 µg/ml

    Lane 1 : Human brain lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 32 kDa

References

ab223665 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223665.
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