Overview

  • Product name
    Anti-EB3 antibody [EPR11421(B)]
    See all EB3 primary antibodies
  • Description
    Rabbit monoclonal [EPR11421(B)] to EB3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EB3. The exact sequence is proprietary.

  • Positive control
    • WB: Human cerebellum, human fetal brain and rat muscle lysates. IHC-P: Human brain tissue, Human skeletal muscle tissue. Flow Cyt: U87 MG cells. ICC/IF: SH-SY5Y cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Predicted molecular weight: 32 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
    • Tissue specificity
      Predominantly expressed in brain and muscle.
    • Sequence similarities
      Belongs to the MAPRE family.
      Contains 1 CH (calponin-homology) domain.
      Contains 1 EB1 C-terminal domain.
    • Domain
      Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
    • Cellular localization
      Cytoplasm > cytoskeleton. Associated with the microtubule network.
    • Information by UniProt
    • Database links
    • Alternative names
      • APC binding protein antibody
      • EB 3 antibody
      • EB1 protein family member 3 antibody
      • EB3 antibody
      • EBF 3 antibody
      • EBF-3 antibody
      • EBF3 antibody
      • EBF3 S antibody
      • End binding protein 3 antibody
      • End-binding protein 3 antibody
      • MAPRE 3 antibody
      • MAPRE3 antibody
      • MARE3_HUMAN antibody
      • Member 3 antibody
      • Microtubule Associated Protein antibody
      • Microtubule associated protein RP/EB family member 3 antibody
      • Microtubule-associated protein RP/EB family member 3 antibody
      • OTTHUMP00000200910 antibody
      • RP 3 antibody
      • RP/EB Family antibody
      • RP3 antibody
      see all

    Images

    • All lanes : Anti-EB3 antibody [EPR11421(B)] (ab157217) at 1/10000 dilution

      Lane 1 : Human cerebellum lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : Rat muscle lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 32 kDa

    • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling EB3 with purified ab157217 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Control: PBS only.

    • Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue labeling EB3 with ab157217 at 1/250.

    • Flow Cytometry analysis of U87-MG (human glioblastoma) cells labeling EB3 with unpurified ab157217 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Immunohistochemical analysis of paraffin embedded human brain tissue labeling EB3 with ab157217 at 1/250.

    References

    This product has been referenced in:
    • Sayas CL  et al. Role of tau N-terminal motif in the secretion of human tau by End Binding proteins. PLoS One 14:e0210864 (2019). Read more (PubMed: 30668577) »
    • Berges R  et al. End-binding 1 protein overexpression correlates with glioblastoma progression and sensitizes to Vinca-alkaloids in vitro and in vivo. Oncotarget 5:12769-87 (2014). Read more (PubMed: 25473893) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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