Recombinant Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)
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Overview
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Product name
Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free
See all EB3 primary antibodies -
Description
Rabbit monoclonal [EPR11421(B)] to EB3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human EB3. The exact sequence is proprietary.
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Positive control
- WB: Human cerebellum, human fetal brain and rat muscle lysates. IHC-P: Human brain tissue, Human skeletal muscle tissue. Flow Cyt: U87 MG cells. ICC/IF: SH-SY5Y cells.
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General notes
Ab246357 is the carrier-free version of ab157217. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab246357 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11421(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab246357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 31 kDa. |
Target
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Function
May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. -
Tissue specificity
Predominantly expressed in brain and muscle. -
Sequence similarities
Belongs to the MAPRE family.
Contains 1 CH (calponin-homology) domain.
Contains 1 EB1 C-terminal domain. -
Domain
Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1. -
Cellular localization
Cytoplasm > cytoskeleton. Associated with the microtubule network. - Information by UniProt
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Database links
- Entrez Gene: 22924 Human
- Entrez Gene: 100732 Mouse
- Entrez Gene: 298848 Rat
- Omim: 605788 Human
- SwissProt: Q9UPY8 Human
- SwissProt: Q6PER3 Mouse
- SwissProt: Q5XIT1 Rat
- Unigene: 515860 Human
see all -
Alternative names
- APC binding protein antibody
- EB 3 antibody
- EB1 protein family member 3 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)](https://www.abcam.com/ps/products/246/ab246357/Images/ab246357-5-ab157217266090ab157217SHSY5Y500.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling EB3 with purified ab157217 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
Control: PBS only.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab157217).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)](https://www.abcam.com/ps/products/246/ab246357/Images/ab246357-3-EB3Primaryantibodiesab1572173.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue labeling EB3 with ab157217 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab157217).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)](https://www.abcam.com/ps/products/246/ab246357/Images/ab246357-2-EB3Primaryantibodiesab1572172.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)Immunohistochemical analysis of paraffin embedded human brain tissue labeling EB3 with ab157217 at 1/250.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab157217).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)](https://www.abcam.com/ps/products/246/ab246357/Images/ab246357-1-ab157217272315ab157217FC1.jpg)
Flow Cytometry - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)Flow Cytometry analysis of U87-MG (human glioblastoma) cells labeling EB3 with unpurified ab157217 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab157217).
Datasheets and documents
References
ab246357 has not yet been referenced specifically in any publications.
