RecombinantRabMAb

Recombinant Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11421(B)] to EB3 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free
    See all EB3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11421(B)] to EB3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human EB3. The exact sequence is proprietary.

  • Positive control

    • WB: Human cerebellum, human fetal brain and rat muscle lysates. IHC-P: Human brain tissue, Human skeletal muscle tissue. Flow Cyt: U87 MG cells. ICC/IF: SH-SY5Y cells.

  • General notes

    Ab246357 is the carrier-free version of ab157217. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab246357 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab246357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.

    • Tissue specificity

      Predominantly expressed in brain and muscle.

    • Sequence similarities

      Belongs to the MAPRE family.
      Contains 1 CH (calponin-homology) domain.
      Contains 1 EB1 C-terminal domain.

    • Domain

      Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.

    • Cellular localization

      Cytoplasm > cytoskeleton. Associated with the microtubule network.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • APC binding protein antibody
      • EB 3 antibody
      • EB1 protein family member 3 antibody
      • EB3 antibody
      • EBF 3 antibody
      • EBF-3 antibody
      • EBF3 antibody
      • EBF3 S antibody
      • End binding protein 3 antibody
      • End-binding protein 3 antibody
      • MAPRE 3 antibody
      • MAPRE3 antibody
      • MARE3_HUMAN antibody
      • Member 3 antibody
      • Microtubule Associated Protein antibody
      • Microtubule associated protein RP/EB family member 3 antibody
      • Microtubule-associated protein RP/EB family member 3 antibody
      • OTTHUMP00000200910 antibody
      • RP 3 antibody
      • RP/EB Family antibody
      • RP3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)
      Immunocytochemistry/ Immunofluorescence - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)

      Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling EB3 with purified ab157217 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Control: PBS only.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab157217).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)

      Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue labeling EB3 with ab157217 at 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab157217).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)

      Immunohistochemical analysis of paraffin embedded human brain tissue labeling EB3 with ab157217 at 1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab157217).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)
      Flow Cytometry - Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)

      Flow Cytometry analysis of U87-MG (human glioblastoma) cells labeling EB3 with unpurified ab157217 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab157217).

    • Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)
      Anti-EB3 antibody [EPR11421(B)] - BSA and Azide free (ab246357)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab246357? Please let us know so that we can cite the reference in this datasheet.

    ab246357 has not yet been referenced specifically in any publications.

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