• Product name

    Anti-EB3 antibody [KT36]
    See all EB3 primary antibodies
  • Description

    Rat monoclonal [KT36] to EB3
  • Host species

  • Tested applications

    Suitable for: ELISA, ICC/IF, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length human EB3 fused to a tag, purified from E. coli BL21 bacteria.



Our Abpromise guarantee covers the use of ab53360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
ICC/IF 1/100 - 1/1000.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.



  • Function

    May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • Tissue specificity

    Predominantly expressed in brain and muscle.
  • Sequence similarities

    Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • Domain

    Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • Cellular localization

    Cytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt
  • Database links

  • Alternative names

    • APC binding protein antibody
    • EB 3 antibody
    • EB1 protein family member 3 antibody
    • EB3 antibody
    • EBF 3 antibody
    • EBF-3 antibody
    • EBF3 antibody
    • EBF3 S antibody
    • End binding protein 3 antibody
    • End-binding protein 3 antibody
    • MAPRE 3 antibody
    • MAPRE3 antibody
    • MARE3_HUMAN antibody
    • Member 3 antibody
    • Microtubule Associated Protein antibody
    • Microtubule associated protein RP/EB family member 3 antibody
    • Microtubule-associated protein RP/EB family member 3 antibody
    • OTTHUMP00000200910 antibody
    • RP 3 antibody
    • RP/EB Family antibody
    • RP3 antibody
    see all


  • Anti-EB3 antibody [KT36] (ab53360) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 32 kDa

    Exposure time: 8 minutes
  • Immunofluorescent staining for COS7 cells overexpressing EB3, using ab53360 at 1/100.
  • Immunofluorescent staining of cell mitosis using ab53360 crude culture supernatant diluted 1:10 (Please note that there was about 50-100 ug/ml antibody in the supernatants) COS7 Transformed African Green Monkey Kidney Fibroblast Cells.
  • Overlay histogram showing SH-SY5Y cells stained with ab53360 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53360, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:

  • Pavez M  et al. STIM1 is required for remodelling of the endoplasmic reticulum and microtubule cytoskeleton in steering growth cones. J Neurosci N/A:N/A (2019). Read more (PubMed: 31023836) »
  • Fassier C  et al. Motor axon navigation relies on Fidgetin-like 1-driven microtubule plus end dynamics. J Cell Biol 217:1719-1738 (2018). Read more (PubMed: 29535193) »
See all 7 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Mouse Cell (Primary forebrain neurons)
Primary forebrain neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C

Jacob Garza

Verified customer

Submitted Jan 29 2018

Western blot
Mouse Tissue lysate - whole (Brain-fresh dissected hippocampus and lysed with p)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
25 µg
Brain-fresh dissected hippocampus and lysed with p
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Klaudio Valued Customer

Verified customer

Submitted May 15 2015

Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Mouse Cell (primary hippocampal neurons)
primary hippocampal neurons
Yes - 0.1% Triton X-100

Abcam user community

Verified customer

Submitted Sep 25 2013

Immunocytochemistry/ Immunofluorescence
Rat Cell (Rat-2 and cultured rat hippocampal neurons)
Rat-2 and cultured rat hippocampal neurons
Yes - Triton X100

Mrs. Marcelina Pieprzyk

Verified customer

Submitted Feb 05 2013


Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to confirm you that ab53360 has not been tested for use in mouse samples and therefore is not covered by our 6 month guarantee.

Reviewing this case, I would like to offer some suggestions to help optimise the results from ab53360.

1.) Some epitopes are very sensitive to methanol as it can disrupt epitope structure. Can try acetone instead for permeabilization if required or use 4% PFA and a permeabilisation agent.

2.) I suggest to use 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions.

3.) I Since the secondary antibody alone give high background, I strongly recommend to use a duifferent antibody. Since mouse and rat are very closely related, I suggest to use a secondary antibody that is pre-absorped against cross-reactivity to rat.

I would also appreciate if you can confirm some further details:

Please send an image of your results, this will help us to provide suitable tips.

I hope this information is helpful, thank you for your cooperation.

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Thank you for your reply.

Thats no problem. However, if you would like to participate in this scheme please do let us know prior to purchasing the antibody. I have generated the discount code as requested:

Discount code: xxxxxxx

Expiration date: 19th of December 2012

This code will give you: 1 freeprimary antibody or the value of ab53360off any product in our catalogue before the expiration date above. To redeem this offer, please submit an Abreview formouse and/or ratand include this code in the “Additional Comments” section so we know the Abreview is for this promotion.

Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site:


We publish both positive and negative Abreviews on our datasheets so please submit the results of your tests, whatever you find. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:


Read More


Thank you for your reply.

The immunogen used to raise the anti-EB3 antibody [KT36] (ab53360) was of the recombinantly expressed full length human EB3 protein. This shares 99% homology with the rat and mouse protein. We would therefore predict that the antibody would cross react. However, as wehave not tested this ourselves as yet we would not be able to guarantee this.

However, if you would like to purchase ab53360 and use it with yourmouse and ratsamples, we can offer you a testing discount. This offer means that if you purchase ab53360 as normal, test the antibody with yoursamples and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab53360 off any product). More information on this offer can be found here:


Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab53360.

Alternatively, you may want to have a look at the rabbit polyclonal ab13859 which we have shown is able to detect the human, rat and mouse protein.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More


Thank you for contacting us and your interest in our products.

As yet, we have not tested the Anti-EB3 antibody [KT36] (ab53360) in any rodent species. However, this does not mean that the antibody would not work. As the immunogen used to raise the antibody, full length human EB3 fused to a tag, shares 99% homology with the mouse and rat protein, we would expect the antibody to be able to detect these proteins. As we have not tested it we would not be able to guarantee it.

If you can describe the experiment you are hoping to perform (which type of samples and application)I can see if I would recommend trying this antibody or if we have any other antibodies against this target which may be more suitable.

I look forward to receiving your reply.

Read More

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