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    ebf1-antibody-ab126135.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-EBF1 antibody (ab126135)

  • Datasheet
  • SDS
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Western blot - Anti-EBF1 antibody (ab126135)

    Key features and details

    • Rabbit polyclonal to EBF1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-EBF1 antibody
      See all EBF1 primary antibodies
    • Description

      Rabbit polyclonal to EBF1
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Chicken, Cow
    • Immunogen

      Recombinant fragment, corresponding to a region within amino acids 57-317 of Human EBF1.

    • Positive control

      • Purchase matching WB positive control:Recombinant Human EBF1 protein
      • THP-1 whole cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • THP-1 whole cell lysate (ab7913)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab126135 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 64 kDa.
    Notes
    WB
    1/500 - 1/3000. Predicted molecular weight: 64 kDa.

    Target

    • Function

      Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
    • Sequence similarities

      Belongs to the COE family.
      Contains 1 IPT/TIG domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9UH73 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 395512 Chicken
      • Entrez Gene: 537712 Cow
      • Entrez Gene: 1879 Human
      • Entrez Gene: 13591 Mouse
      • Entrez Gene: 116543 Rat
      • Omim: 164343 Human
      • SwissProt: Q9UH73 Human
      • SwissProt: Q07802 Mouse
      • SwissProt: Q63398 Rat
      • Unigene: 573143 Human
      • Unigene: 439662 Mouse
      • Unigene: 440346 Mouse
      • Unigene: 11257 Rat
      see all
    • Alternative names

      • COE1 antibody
      • COE1_HUMAN antibody
      • Collier, Olf and EBF transcription factor 1 antibody
      • Early B cell factor 1 antibody
      • Early B-cell factor antibody
      • EBF antibody
      • Ebf1 antibody
      • O/E 1 antibody
      • O/E-1 antibody
      • OE 1 antibody
      • OE-1 antibody
      • OE1 antibody
      • OLF1 antibody
      • olfactory neuronal transcription factor 1 antibody
      • Transcription factor COE1 antibody
      see all

    Images

    • Western blot - Anti-EBF1 antibody (ab126135)
      Western blot - Anti-EBF1 antibody (ab126135)
      Anti-EBF1 antibody (ab126135) at 1/1000 dilution + THP-1 whole cell lysate at 30 µg

      Performed under reducing conditions.

      Predicted band size: 64 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab126135? Please let us know so that we can cite the reference in this datasheet.

    ab126135 has been referenced in 1 publication.

    • Yamada Y  et al. Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation. Int J Mol Med 33:1355-63 (2014). PubMed: 24626634

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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