Anti-EBF1 antibody (ab126135)
Key features and details
- Rabbit polyclonal to EBF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EBF1 antibody
See all EBF1 primary antibodies -
Description
Rabbit polyclonal to EBF1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 57-317 of Human EBF1.
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Positive control
- Purchase matching WB positive control:Recombinant Human EBF1 protein
- THP-1 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 64 kDa.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 64 kDa. |
Target
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Function
Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. -
Sequence similarities
Belongs to the COE family.
Contains 1 IPT/TIG domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395512 Chicken
- Entrez Gene: 537712 Cow
- Entrez Gene: 1879 Human
- Entrez Gene: 13591 Mouse
- Entrez Gene: 116543 Rat
- Omim: 164343 Human
- SwissProt: Q9UH73 Human
- SwissProt: Q07802 Mouse
see all -
Alternative names
- COE1 antibody
- COE1_HUMAN antibody
- Collier, Olf and EBF transcription factor 1 antibody
see all
Images
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Western blot - Anti-EBF1 antibody (ab126135)Anti-EBF1 antibody (ab126135) at 1/1000 dilution + THP-1 whole cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 64 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab126135 has been referenced in 1 publication.
- Yamada Y et al. Identification of hypo- and hypermethylated genes related to atherosclerosis by a genome-wide analysis of DNA methylation. Int J Mol Med 33:1355-63 (2014). PubMed: 24626634