Product nameAnti-EBF1 antibody
See all EBF1 primary antibodies
DescriptionGoat polyclonal to EBF1
SpecificityThis antibody is expected not to cross-react with EBF2, EBF3 and EBF4.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken
- Purchase matching WB positive control:Recombinant Human EBF1 protein
- Human adipose lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85689 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab85689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
1 hour primary incubation is recommended for this product.
FunctionTranscriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.
Sequence similaritiesBelongs to the COE family.
Contains 1 IPT/TIG domain.
- Information by UniProt
- COE1 antibody
- COE1_HUMAN antibody
- Collier, Olf and EBF transcription factor 1 antibody
Anti-EBF1 antibody (ab85689) at 0.3 µg/ml + Human adipose lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 64 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab85689 has not yet been referenced specifically in any publications.