Anti-EBI3 antibody [DNT27] (ab118500)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [DNT27] (ab118500)](https://www.abcam.com/ps/products/118/ab118500/Images/ab118500-130940-anti-ebi3-antibody-dnt27-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [DNT27] (ab118500)")
Key features and details
- Rat monoclonal [DNT27] to EBI3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-EBI3 antibody [DNT27]
See all EBI3 primary antibodies -
Description
Rat monoclonal [DNT27] to EBI3 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Recombinant fragment
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Immunogen
Full length Mouse EBI3
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Positive control
- Human Lyphoid-Dendritic Cells, Recombinant mouse EBI3/p28 (covalently linked IL-27) protein, mouse bone marrow-derived dendritic cells stimulated with LPS
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Aqueous buffer 99% -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
DNT27 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118500 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Recombinant fragment
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.
|
| IHC-P | (2) |
Use a concentration of 10 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa. |
|
IHC-P
Use a concentration of 10 µg/ml. |
Target
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Function
Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 2 fibronectin type-III domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 10148 Human
- Entrez Gene: 50498 Mouse
- Omim: 605816 Human
- SwissProt: Q14213 Human
- SwissProt: O35228 Mouse
- Unigene: 501452 Human
- Unigene: 256798 Mouse
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Alternative names
- cytokine receptor antibody
- EBI3 antibody
- EBV induced gene 3 protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [DNT27] (ab118500)ab119500 at 10 µg/ml staining EBI3 in Human Lyphoid-Dendritic Cells by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
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![Western blot - Anti-EBI3 antibody [DNT27] (ab118500)](https://www.abcam.com/ps/products/118/ab118500/Images/ab118500-130939-anti-ebi3-antibody-dnt27-western-blot.jpg)
Western blot - Anti-EBI3 antibody [DNT27] (ab118500)All lanes : Anti-EBI3 antibody [DNT27] (ab118500) at 5 µg/ml
Lane 1 : Recombinant mouse EBI3/p28 (covalently linked IL-27) protein
Lane 2 : Mouse bone marrow-derived dendritic cells stimulated with LPS
Lane 3 : Untreated
Secondary
All lanes : Horseradish peroxidase (HRP)-conjugated goat anti-rat IgG
Predicted band size: 25 kDa
Protocols
Datasheets and documents
References (1)
ab118500 has been referenced in 1 publication.
- Terayama H et al. Contribution of IL-12/IL-35 common subunit p35 to maintaining the testicular immune privilege. PLoS One 9:e96120 (2014). IHC-Fr ; Mouse . PubMed: 24760014
