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    ebi3-antibody-dnt27-ab118500.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-EBI3 antibody [DNT27] (ab118500)

  • Datasheet
Reviews (4) Submit a question References (1)

Product price, shipping and contact information

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [DNT27] (ab118500)
  • Western blot - Anti-EBI3 antibody [DNT27] (ab118500)

Key features and details

  • Rat monoclonal [DNT27] to EBI3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG2a

You may also be interested in

Secondary
Product image
Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
Protein
Product image
Recombinant Human EBI3 protein (ab83026)

View more associated products

Overview

  • Product name

    Anti-EBI3 antibody [DNT27]
    See all EBI3 primary antibodies
  • Description

    Rat monoclonal [DNT27] to EBI3
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Full length Mouse EBI3

  • Positive control

    • Human Lyphoid-Dendritic Cells, Recombinant mouse EBI3/p28 (covalently linked IL-27) protein, mouse bone marrow-derived dendritic cells stimulated with LPS

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide

    Aqueous buffer 99%
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    DNT27
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Recombinant Protein

    • Recombinant Human EBI3 protein (ab83026)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab118500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Recombinant fragment
Application Abreviews Notes
WB (2)
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P (2)
Use a concentration of 10 µg/ml.
Notes
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P
Use a concentration of 10 µg/ml.

Target

  • Function

    Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
  • Sequence similarities

    Belongs to the type I cytokine receptor family. Type 3 subfamily.
    Contains 2 fibronectin type-III domains.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q14213 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10148 Human
    • Entrez Gene: 50498 Mouse
    • Omim: 605816 Human
    • SwissProt: Q14213 Human
    • SwissProt: O35228 Mouse
    • Unigene: 501452 Human
    • Unigene: 256798 Mouse
    • Alternative names

      • cytokine receptor antibody
      • EBI3 antibody
      • EBV induced gene 3 protein antibody
      • EBV-induced gene 3 protein antibody
      • Epstein Barr virus induced 3 antibody
      • Epstein Barr virus induced gene 3 antibody
      • Epstein Barr virus induced gene 3 protein antibody
      • Epstein-Barr virus-induced gene 3 protein antibody
      • IL 27 subunit beta antibody
      • IL 27B antibody
      • IL-27 subunit beta antibody
      • IL-27B antibody
      • IL27 subunit antibody
      • IL27 subunit beta antibody
      • IL27B antibody
      • IL27B_HUMAN antibody
      • IL35 subunit antibody
      • interleukin 27 subunit beta antibody
      • Interleukin-27 subunit beta antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [DNT27] (ab118500)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EBI3 antibody [DNT27] (ab118500)
      ab119500 at 10 µg/ml staining EBI3 in Human Lyphoid-Dendritic Cells by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
    • Western blot - Anti-EBI3 antibody [DNT27] (ab118500)
      Western blot - Anti-EBI3 antibody [DNT27] (ab118500)
      All lanes : Anti-EBI3 antibody [DNT27] (ab118500) at 5 µg/ml

      Lane 1 : Recombinant mouse EBI3/p28 (covalently linked IL-27) protein
      Lane 2 : Mouse bone marrow-derived dendritic cells stimulated with LPS
      Lane 3 : Untreated

      Secondary
      All lanes : Horseradish peroxidase (HRP)-conjugated goat anti-rat IgG

      Predicted band size: 25 kDa

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab118500? Please let us know so that we can cite the reference in this datasheet.

    ab118500 has been referenced in 1 publication.

    • Terayama H  et al. Contribution of IL-12/IL-35 common subunit p35 to maintaining the testicular immune privilege. PLoS One 9:e96120 (2014). IHC-Fr ; Mouse . PubMed: 24760014

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EBI3 antibody [DNT27]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate 70C 2hr
    Permeabilization
    No
    Specification
    Testis
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 15 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EBI3 antibody [DNT27]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 19 2017

    Western blot abreview for Anti-EBI3 antibody [DNT27]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse spleen whole extract)
    Loading amount
    100 µg
    Specification
    Mouse spleen whole extract
    Gel Running Conditions
    Reduced Denaturing (10% Tris-glycine gel)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 26 2012

    Western blot abreview for Anti-EBI3 antibody [DNT27]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver whole extract)
    Loading amount
    100 µg
    Specification
    Liver whole extract
    Gel Running Conditions
    Reduced Denaturing (14% Tris-glycine gel)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 21 2012

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