Overview

  • Product name

    Anti-EBI3 antibody [EPR5747]
    See all EBI3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5747] to EBI3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EBI3 aa 200 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • Rat and Human spleen, Human tonsil,K562 and JAR cell lysates; permeabilized K562 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5747
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.
    • Sequence similarities

      Belongs to the type I cytokine receptor family. Type 3 subfamily.
      Contains 2 fibronectin type-III domains.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • cytokine receptor antibody
      • EBI3 antibody
      • EBV induced gene 3 protein antibody
      • EBV-induced gene 3 protein antibody
      • Epstein Barr virus induced 3 antibody
      • Epstein Barr virus induced gene 3 antibody
      • Epstein Barr virus induced gene 3 protein antibody
      • Epstein-Barr virus-induced gene 3 protein antibody
      • IL 27 subunit beta antibody
      • IL 27B antibody
      • IL-27 subunit beta antibody
      • IL-27B antibody
      • IL27 subunit antibody
      • IL27 subunit beta antibody
      • IL27B antibody
      • IL27B_HUMAN antibody
      • IL35 subunit antibody
      • interleukin 27 subunit beta antibody
      • Interleukin-27 subunit beta antibody
      see all

    Images

    • All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified)

      Lane 1 : Mouse kidney tissue lysate
      Lane 2 : Mouse spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified)

      Lane 1 : Rat kidney tissue lysate
      Lane 2 : Rat spleen tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP anti-rabbit, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified) + Human tonsil tissue lysate at 10 µg

      Secondary
      HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/5000 dilution (purified)

      Lane 1 : K562 cell lysate
      Lane 2 : JAR cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-EBI3 antibody [EPR5747] (ab124694) at 1/10000 dilution (unpurified)

      Lane 1 : Rat spleen tissue lysate
      Lane 2 : Human tonsil tissue lysate
      Lane 3 : K562 cell lysate
      Lane 4 : JAR cell lysate
      Lane 5 : Human spleen tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 25 kDa

    References

    This product has been referenced in:

    • Zhang Y  et al. The Unknown Aspect of BAFF: Inducing IL-35 Production by a CD5+CD1dhiFc?RIIbhiRegulatory B-Cell Subset in Lupus. J Invest Dermatol 137:2532-2543 (2017). WB . Read more (PubMed: 28844943) »
    • Wang WJ  et al. Regulation of the expression of Th17 cells and regulatory T cells by IL-27 in patients with unexplained early recurrent miscarriage. J Reprod Immunol 99:39-45 (2013). WB ; Human . Read more (PubMed: 23731956) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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