Overview

  • Product name

    Anti-EBNA 3A antibody
  • Description

    Sheep polyclonal to EBNA 3A
  • Host species

    Sheep
  • Specificity

    This antibody is specific for the Epstein Barr Virus Nuclear Antigen 3A.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Full length viral protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.08% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Purification notes

    Purified by ammonium sulfate precipitation. This antibody is provided as a 0.2µm sterile filtered solution.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab16126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22432076
WB Use a concentration of 0.25 - 1 µg/ml.

Target

  • Relevance

    EBNA3A is a latent viral nuclear protein expressed in Epstein Barr Virus (EBV) transformed lymphoblastic cell lines. It is also found in some immunoblastic lymphomas in vivo. This viral nuclear protein is essential for EBV mediated transformation of B lymphocytes. The EBNA3A functions as a transcriptional regulator though the target genes are currently unknown.
  • Alternative names

    • EBNA 3A antibody
    • EBV antibody
    • Epstein Barr virus antibody
    • HHV4 antibody
    • Nuclear antigen EBNA 3A antibody
    see all

References

This product has been referenced in:

  • Paschos K  et al. Requirement for PRC1 subunit BMI1 in host gene activation by Epstein-Barr virus protein EBNA3C. Nucleic Acids Res N/A:N/A (2019). Read more (PubMed: 30649516) »
  • Gillman ACT  et al. Epstein-Barr Virus Nuclear Antigen 3C Inhibits Expression of COBLL1 and the ADAM28-ADAMDEC1 Locus via Interaction with the Histone Lysine Demethylase KDM2B. J Virol 92:N/A (2018). Read more (PubMed: 30135119) »
See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your response.

As the datasheet indicates, the immunogen used to raise ab16126 was a full length viral protein. Since this is a polyclonal antibody, it recognizes multiple epitopes. For polyclonals, epitope mapping is not carried out so we do not have any information about the region it binds to.


If you need any further assistance in the future, please do not hesitate to contact me.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (Burkitt's lymphoma line)
Loading amount
50000 cells
Specification
Burkitt's lymphoma line
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

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Verified customer

Submitted May 19 2010

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