Key features and details
- Sheep polyclonal to EBNA 3A
- Suitable for: ICC, Dot blot, ELISA, IHC-Fr, WB
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-EBNA 3A antibody
DescriptionSheep polyclonal to EBNA 3A
SpecificityThis antibody is specific for the Epstein Barr Virus Nuclear Antigen 3A.
Tested applicationsSuitable for: ICC, Dot blot, ELISA, IHC-Fr, WBmore details
Species reactivityReacts with: Species independent
Recombinant full length protein. Recombinant EBNA-3A protein
- WB: EBV infected tissues and cells
Sheep were immunized with recombinant EBNA-3A protein
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.08% Sodium azide
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesPurified by ammonium sulfate precipitation. This antibody is provided as a 0.2µm sterile filtered solution.
Our Abpromise guarantee covers the use of ab16126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.25 - 1 µg/ml.|
RelevanceEBNA3A is a latent viral nuclear protein expressed in Epstein Barr Virus (EBV) transformed lymphoblastic cell lines. It is also found in some immunoblastic lymphomas in vivo. This viral nuclear protein is essential for EBV mediated transformation of B lymphocytes. The EBNA3A functions as a transcriptional regulator though the target genes are currently unknown.
- EBNA 3A antibody
- EBV antibody
- Epstein Barr virus antibody
ab16126 has been referenced in 9 publications.
- Paschos K et al. Requirement for PRC1 subunit BMI1 in host gene activation by Epstein-Barr virus protein EBNA3C. Nucleic Acids Res N/A:N/A (2019). PubMed: 30649516
- Gillman ACT et al. Epstein-Barr Virus Nuclear Antigen 3C Inhibits Expression of COBLL1 and the ADAM28-ADAMDEC1 Locus via Interaction with the Histone Lysine Demethylase KDM2B. J Virol 92:N/A (2018). PubMed: 30135119
- Szymula A et al. Epstein-Barr virus nuclear antigen EBNA-LP is essential for transforming naïve B cells, and facilitates recruitment of transcription factors to the viral genome. PLoS Pathog 14:e1006890 (2018). PubMed: 29462212
- Bazot Q et al. Epstein-Barr Virus (EBV) Latent Protein EBNA3A Directly Targets and Silences the STK39 Gene in B Cells Infected by EBV. J Virol 92:N/A (2018). PubMed: 29367247
- McHugh D et al. Persistent KSHV Infection Increases EBV-Associated Tumor Formation In Vivo via Enhanced EBV Lytic Gene Expression. Cell Host Microbe 22:61-73.e7 (2017). PubMed: 28704654
- Bazot Q et al. Epstein-Barr Virus Proteins EBNA3A and EBNA3C Together Induce Expression of the Oncogenic MicroRNA Cluster miR-221/miR-222 and Ablate Expression of Its Target p57KIP2. PLoS Pathog 11:e1005031 (2015). PubMed: 26153983
- Quan L et al. PD-1 Blockade Can Restore Functions of T-Cells in Epstein-Barr Virus-Positive Diffuse Large B-Cell Lymphoma In Vitro. PLoS One 10:e0136476 (2015). IHC-P ; Human . PubMed: 26361042
- Nguyen-Van D et al. Epstein-Barr virus-positive diffuse large B-cell lymphoma of the elderly expresses EBNA3A with conserved CD8 T-cell epitopes. Am J Blood Res 1:146-59 (2011). IHC-P ; Human . PubMed: 22432076
- Sun X et al. Hsp90 inhibitors block outgrowth of EBV-infected malignant cells in vitro and in vivo through an EBNA1-dependent mechanism. Proc Natl Acad Sci U S A 107:3146-51 (2010). WB . PubMed: 20133771