Overview

  • Product name
    Anti-EBNA2 antibody [PE2]
    See all EBNA2 primary antibodies
  • Description
    Mouse monoclonal [PE2] to EBNA2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Epstein Barr Virus.
  • Immunogen

    Recombinant fusion protein against EBNA2.

Properties

Applications

Our Abpromise guarantee covers the use of ab90543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    The Epstein-Barr Virus (EBV), also called Human herpesvirus 4 (HHV-4), is a virus of the herpes family and is one of the most common viruses in humans. EBNA2 is one of the few genes of Epstein-Barr virus which are necessary for immortalization of human primary B lymphocytes. The EBNA2 protein acts as a transcriptional activator of several viral and cellular genes.
  • Cellular localization
    Nuclear matrix of host cells
  • Alternative names
    • EBNA2 antibody
    • EBV antibody
    • EBV nuclear antigen 2 antibody
    • Epstein Barr nuclear antigen 2 antibody
    • HHV4 antibody
    • Human Herpesvirus 4 antibody
    see all

References

This product has been referenced in:
  • Szymula A  et al. Epstein-Barr virus nuclear antigen EBNA-LP is essential for transforming naïve B cells, and facilitates recruitment of transcription factors to the viral genome. PLoS Pathog 14:e1006890 (2018). Read more (PubMed: 29462212) »
  • Gion Y  et al. Clinicopathological analysis of methotrexate-associated lymphoproliferative disorders: Comparison of diffuse large B-cell lymphoma and classical Hodgkin lymphoma types. Cancer Sci 108:1271-1280 (2017). IHC-P ; Human . Read more (PubMed: 28380678) »

See all 15 Publications for this product

Customer reviews and Q&As

Brink et al. 1997. J Clin Pathol. 50(11):911-8. PMID: http://www.ncbi.nlm.nih.gov/pubmed/?term=brink+et+al+1997+j+clin+pathol+50(11)%3A911-8 (http://www.ncbi.nlm.nih.gov/pubmed/?term=brink+et+al+1997+j+clin+pathol+50(11)%3A911-8). Suggests that clone P...

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0 Bond Epitope Retrieval Solution 1 (ER1, Leica)
Sample
Rhesus monkey Tissue sections (Esophagus)
Specification
Esophagus
Permeabilization
No
Fixative
10% Neutral Buffered Formalin
Username

Abcam user community

Verified customer

Submitted Mar 16 2015

The epitope is between aa 405-480 of EBNA2.

Thank you for contacting us. As far as we are aware, ab90543 has never been tested in IHC-P. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention. It would be ve...

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Thank you for contacting us. The secondary antibody ab6785 (goat anti-mouse IgG, FITC-conjugated) is a suitable secondary for the three primary antibodies you asked about (ab78113, ab90543, and ab3280), as well as ab6276. I hope this inf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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