Anti-EBNA2 antibody [PE2] (ab90543)

Mouse monoclonal EBNA2 antibody [PE2]. Validated in WB, IP, IHC, ICC/IF. Cited in 17 publication(s). Independently reviewed in 1 review(s).

Overview

  • Product name

    Anti-EBNA2 antibody [PE2]
    See all EBNA2 primary antibodies
  • Description

    Mouse monoclonal [PE2] to EBNA2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant fusion protein against EBNA2.

Properties

Applications

Our Abpromise guarantee covers the use of ab90543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    The Epstein-Barr Virus (EBV), also called Human herpesvirus 4 (HHV-4), is a virus of the herpes family and is one of the most common viruses in humans. EBNA2 is one of the few genes of Epstein-Barr virus which are necessary for immortalization of human primary B lymphocytes. The EBNA2 protein acts as a transcriptional activator of several viral and cellular genes.
  • Cellular localization

    Nuclear matrix of host cells
  • Alternative names

    • EBNA2 antibody
    • EBV antibody
    • EBV nuclear antigen 2 antibody
    • Epstein Barr nuclear antigen 2 antibody
    • HHV4 antibody
    • Human Herpesvirus 4 antibody
    see all

References

This product has been referenced in:

  • Szymula A  et al. Epstein-Barr virus nuclear antigen EBNA-LP is essential for transforming naïve B cells, and facilitates recruitment of transcription factors to the viral genome. PLoS Pathog 14:e1006890 (2018). Read more (PubMed: 29462212) »
  • Bristol JA  et al. A cancer-associated Epstein-Barr virus BZLF1 promoter variant enhances lytic infection. PLoS Pathog 14:e1007179 (2018). Read more (PubMed: 30052684) »
See all 19 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question
Answer

Brink et al. 1997. J Clin Pathol. 50(11):911-8. PMID: http://www.ncbi.nlm.nih.gov/pubmed/?term=brink+et+al+1997+j+clin+pathol+50(11)%3A911-8 (http://www.ncbi.nlm.nih.gov/pubmed/?term=brink+et+al+1997+j+clin+pathol+50(11)%3A911-8). Suggests that clone PE2 recognises EBNA2 from EBV types 1 and 2.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0 Bond Epitope Retrieval Solution 1 (ER1, Leica)
Sample
Rhesus monkey Tissue sections (Esophagus)
Specification
Esophagus
Permeabilization
No
Fixative
10% Neutral Buffered Formalin

Abcam user community

Verified customer

Submitted Mar 16 2015

Question
Answer

The epitope is between aa 405-480 of EBNA2.

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Answer

Thank you for contacting us. As far as we are aware, ab90543 has never been tested in IHC-P. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention. It would be very much appreciated if your customer could find this publication again. Could you please ask your customer if it was the specific Abcam antibody reference ab90543 that was used in the publication or if it was the clone [PE2]? If it is the clone, we won't add this publication to our product datasheet since the purity and, more generally, the production process may be different from our own product ab90543. IHC-Fr and IHC-P cannot be grouped onto the same application. Some antibodies would work in both techniques, some would be suitable for either one or the other. If your customer would like to test the antibody in this untested application, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

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Answer

Thank you for contacting us. The secondary antibody ab6785 (goat anti-mouse IgG, FITC-conjugated) is a suitable secondary for the three primary antibodies you asked about (ab78113, ab90543, and ab3280), as well as ab6276. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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