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  1. Link

    ebna2-antibody-pe2-ab90543.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus EBNA
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Anti-EBNA2 antibody [PE2] (ab90543)

  • Datasheet
  • SDS
Reviews (1)Q&A (4)References (26)

Product price, shipping and contact information

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Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Mouse monoclonal [PE2] to EBNA2
  • Suitable for: WB, IP, IHC-Fr, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG1

You may also be interested in

Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Protein
Product image
Recombinant EBNA2 protein (Tagged) (ab241243)
Primary
Anti-EBV Latent Membrane Protein 1 antibody [CS 1-4] - BSA and Azide free (ab78113)

View more associated products

Overview

  • Product name

    Anti-EBNA2 antibody [PE2]
    See all EBNA2 primary antibodies
  • Description

    Mouse monoclonal [PE2] to EBNA2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Recombinant fusion protein against EBNA2.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    PE2
  • Myeloma

    x63-Ag8.653
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • EBNA
    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Epstein Barr

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant EBNA2 protein (Tagged) (ab241243)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab90543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
IP
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

Target

  • Relevance

    The Epstein-Barr Virus (EBV), also called Human herpesvirus 4 (HHV-4), is a virus of the herpes family and is one of the most common viruses in humans. EBNA2 is one of the few genes of Epstein-Barr virus which are necessary for immortalization of human primary B lymphocytes. The EBNA2 protein acts as a transcriptional activator of several viral and cellular genes.
  • Cellular localization

    Nuclear matrix of host cells
  • Alternative names

    • EBNA2 antibody
    • EBV antibody
    • EBV nuclear antigen 2 antibody
    • Epstein Barr nuclear antigen 2 antibody
    • HHV4 antibody
    • Human Herpesvirus 4 antibody
    see all

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (26)

Publishing research using ab90543? Please let us know so that we can cite the reference in this datasheet.

ab90543 has been referenced in 26 publications.

  • Yu F  et al. Characterization and Establishment of a Novel EBV Strain Simultaneously Associated With Nasopharyngeal Carcinoma and B-Cell Lymphoma. Front Oncol 11:626659 (2021). PubMed: 33898307
  • Caduff N  et al. Immunosuppressive FK506 treatment leads to more frequent EBV-associated lymphoproliferative disease in humanized mice. PLoS Pathog 16:e1008477 (2020). PubMed: 32251475
  • McHugh D  et al. EBV renders B cells susceptible to HIV-1 in humanized mice. Life Sci Alliance 3:N/A (2020). PubMed: 32576602
  • Chizhikov D  et al. Effects of Phosphatidylserine Source of Docosahexaenoic Acid on Cerebellar Development in Preterm Pigs. Brain Sci 10:N/A (2020). PubMed: 32718081
  • Chatterjee B  et al. CD8+ T cells retain protective functions despite sustained inhibitory receptor expression during Epstein-Barr virus infection in vivo. PLoS Pathog 15:e1007748 (2019). PubMed: 31145756
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EBNA2 antibody [PE2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0 Bond Epitope Retrieval Solution 1 (ER1, Leica)
Sample
Rhesus monkey Tissue sections (Esophagus)
Specification
Esophagus
Permeabilization
No
Fixative
10% Neutral Buffered Formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 16 2015

Question

Does ab90543 have specificity to EBNA2 of Type 1 or Type 2 EBV?

Read More

Abcam community

Verified customer

Asked on Oct 30 2015

Answer

Brink et al. 1997. J Clin Pathol. 50(11):911-8. PMID: http://www.ncbi.nlm.nih.gov/pubmed/?term=brink+et+al+1997+j+clin+pathol+50(11)%3A911-8 (http://www.ncbi.nlm.nih.gov/pubmed/?term=brink+et+al+1997+j+clin+pathol+50(11)%3A911-8). Suggests that clone PE2 recognises EBNA2 from EBV types 1 and 2.

Read More

Kevin Hanson

Abcam Scientific Support

Answered on Oct 30 2015

Question

Customer kindly inquired as to the immunogen sequence for ab90543

Read More

Abcam community

Verified customer

Asked on Sep 11 2013

Answer

The epitope is between aa 405-480 of EBNA2.

Read More

Kevin Hanson

Abcam Scientific Support

Answered on Sep 11 2013

Question

Dear Technical Team, Our customer purchsed ab90543 because she read ab90543 was used for IHC-P in a paper. However, on the website, the application of this antibody was only for IHC-Fr not IHC-P. 1. Could you double check if this antibody was used for IHC-P (she cannot reamemer the title of the paper..)? 2. If the application is shown as IHC-Fr, should the user not use this antibody for IHC-P? or does it only mean that this application has not been tested? Thank you in advance for your answer.

Read More

Abcam community

Verified customer

Asked on Nov 28 2011

Answer

Thank you for contacting us. As far as we are aware, ab90543 has never been tested in IHC-P. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention. It would be very much appreciated if your customer could find this publication again. Could you please ask your customer if it was the specific Abcam antibody reference ab90543 that was used in the publication or if it was the clone [PE2]? If it is the clone, we won't add this publication to our product datasheet since the purity and, more generally, the production process may be different from our own product ab90543. IHC-Fr and IHC-P cannot be grouped onto the same application. Some antibodies would work in both techniques, some would be suitable for either one or the other. If your customer would like to test the antibody in this untested application, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

Read More

Abcam Scientific Support

Answered on Nov 28 2011

Question

Dear sir\madam, thanks for your cooperation and response. please we also looking for a secondary antibody: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785) and Anti-beta Actin antibody [AC-15] (ab6276). also please let me know if it is compatible with the three previous primary antibodies.

Read More

Abcam community

Verified customer

Asked on Sep 19 2011

Answer

Thank you for contacting us. The secondary antibody ab6785 (goat anti-mouse IgG, FITC-conjugated) is a suitable secondary for the three primary antibodies you asked about (ab78113, ab90543, and ab3280), as well as ab6276. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Sep 19 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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