Overview

  • Product name
    Anti-EBNA2 antibody [PE2]
    See all EBNA2 primary antibodies
  • Description
    Mouse monoclonal [PE2] to EBNA2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IP, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Epstein Barr Virus.
  • Immunogen

    Recombinant fusion protein against EBNA2.

Properties

Applications

Our Abpromise guarantee covers the use of ab90543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    The Epstein-Barr Virus (EBV), also called Human herpesvirus 4 (HHV-4), is a virus of the herpes family and is one of the most common viruses in humans. EBNA2 is one of the few genes of Epstein-Barr virus which are necessary for immortalization of human primary B lymphocytes. The EBNA2 protein acts as a transcriptional activator of several viral and cellular genes.
  • Cellular localization
    Nuclear matrix of host cells
  • Alternative names
    • EBNA2 antibody
    • EBV antibody
    • EBV nuclear antigen 2 antibody
    • Epstein Barr nuclear antigen 2 antibody
    • HHV4 antibody
    • Human Herpesvirus 4 antibody
    see all

References

This product has been referenced in:
  • Szymula A  et al. Epstein-Barr virus nuclear antigen EBNA-LP is essential for transforming naïve B cells, and facilitates recruitment of transcription factors to the viral genome. PLoS Pathog 14:e1006890 (2018). Read more (PubMed: 29462212) »
  • Gion Y  et al. Clinicopathological analysis of methotrexate-associated lymphoproliferative disorders: Comparison of diffuse large B-cell lymphoma and classical Hodgkin lymphoma types. Cancer Sci 108:1271-1280 (2017). IHC-P ; Human . Read more (PubMed: 28380678) »

See all 15 Publications for this product

Publishing research using ab90543? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

5 reviews or Q&A

10
Votes: Highest First

Does ab90543 have specificity to EBNA2 of Type 1 or Type 2 EBV?

Brink et al. 1997. J Clin Pathol. 50(11):911-8. PMID: http://www.ncbi.nlm.nih.gov/pubmed/?term=brink+et+al+1997+j+clin+pathol+50(11)%3A911-8 (http://www.ncbi.nlm.nih.gov/pubmed/?term=brink+et+al+1997+j+clin+pathol+50(11)%3A911-8). Suggests that clone P...

Read More

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EBNA2 antibody [PE2]

 Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
2.5% Normal Horse Serum - VectorLabs ImmPRESS™ Excel Amplified HRP Polymer Staining Kit (Anti-Mouse Ig) as blocking agent for 20 minute(s) · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0 Bond Epitope Retrieval Solution 1 (ER1, Leica)
Sample
Rhesus monkey Tissue sections (Esophagus)
Specification
Esophagus
Permeabilization
No
Fixative
10% Neutral Buffered Formalin
Read More
abreview image
Username

Abcam user community

Verified customer

Submitted Mar 16 2015

Customer kindly inquired as to the immunogen sequence for ab90543

The epitope is between aa 405-480 of EBNA2.

Dear Technical Team, Our customer purchsed ab90543 because she read ab90543 was used for IHC-P in a paper. However, on the website, the application of this antibody was only for IHC-Fr not IHC-P. 1. Could you double check if this ...

Read More

Thank you for contacting us. As far as we are aware, ab90543 has never been tested in IHC-P. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention. It would be ve...

Read More

Dear sir\madam, thanks for your cooperation and response. please we also looking for a secondary antibody: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab6785) and Anti-beta Actin antibody [AC-15] (ab6276). a...

Read More

Thank you for contacting us. The secondary antibody ab6785 (goat anti-mouse IgG, FITC-conjugated) is a suitable secondary for the three primary antibodies you asked about (ab78113, ab90543, and ab3280), as well as ab6276. I hope this inf...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up