Anti-EBP1 antibody (ab137566)
- Datasheet
- References
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Overview
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Product name
Anti-EBP1 antibody
See all EBP1 primary antibodies -
Description
Rabbit polyclonal to EBP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 134-346 of Human EBP1
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Positive control
- 293T, A431, Jurkat, Raji, Mouse brain
Methanol fixed A431 cells
- 293T, A431, Jurkat, Raji, Mouse brain
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General notes
Keep as concentrated solution.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137566 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 44 kDa. |
Target
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Function
May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). -
Tissue specificity
Expressed in several cell lines tested, including primary and transformed cell lines. -
Sequence similarities
Belongs to the peptidase M24 family. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG. - Information by UniProt
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Database links
- Entrez Gene: 540272 Cow
- Entrez Gene: 5036 Human
- Entrez Gene: 18813 Mouse
- Entrez Gene: 288778 Rat
- Omim: 602145 Human
- SwissProt: Q9UQ80 Human
- SwissProt: P50580 Mouse
- Unigene: 524498 Human
see all -
Alternative names
- 38kDa antibody
- AA672939 antibody
- Cell cycle protein p38 2G4 homolog antibody
see all
Images
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Western blot - Anti-EBP1 antibody (ab137566)Anti-EBP1 antibody (ab137566) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Western blot - Anti-EBP1 antibody (ab137566)Anti-EBP1 antibody (ab137566) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody (ab137566)Immunofluorescence analysis of methanol fixed A431 cells, labelling EBP1 with ab137566 at 1/200 dilution (left), costained with Hoechst 33342 (right).
References
ab137566 has not yet been referenced specifically in any publications.
