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    ebp1-antibody-ab137566.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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Anti-EBP1 antibody (ab137566)

  • Datasheet
  • SDS
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Western blot - Anti-EBP1 antibody (ab137566)
  • Western blot - Anti-EBP1 antibody (ab137566)
  • Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody (ab137566)

Key features and details

  • Rabbit polyclonal to EBP1
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human EBP1 protein (ab95350)

View more associated products

Overview

  • Product name

    Anti-EBP1 antibody
    See all EBP1 primary antibodies
  • Description

    Rabbit polyclonal to EBP1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 134-346 of Human EBP1

  • Positive control

    • 293T, A431, Jurkat, Raji, Mouse brain
      Methanol fixed A431 cells
  • General notes

    Keep as concentrated solution.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Ribosome
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • Raji whole cell lysate (ab30124)
    • Mouse brain tissue lysate - total protein (ab30151)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human EBP1 protein (ab95350)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Mouse
Human
All applications
Rat
Cow
Xenopus laevis
Xenopus tropicalis
Application Abreviews Notes
ICC/IF
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa.
Notes
ICC/IF
1/100 - 1/1000.
WB
1/500 - 1/3000. Predicted molecular weight: 44 kDa.

Target

  • Function

    May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site).
  • Tissue specificity

    Expressed in several cell lines tested, including primary and transformed cell lines.
  • Sequence similarities

    Belongs to the peptidase M24 family.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG.
  • Target information above from: UniProt accession Q9UQ80 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 540272 Cow
    • Entrez Gene: 5036 Human
    • Entrez Gene: 18813 Mouse
    • Entrez Gene: 288778 Rat
    • Omim: 602145 Human
    • SwissProt: Q9UQ80 Human
    • SwissProt: P50580 Mouse
    • Unigene: 524498 Human
    • Unigene: 573018 Human
    • Unigene: 4742 Mouse
    • Unigene: 9485 Rat
    see all
  • Alternative names

    • 38kDa antibody
    • AA672939 antibody
    • Cell cycle protein p38 2G4 homolog antibody
    • Cell cycle protein p38-2G4 homolog antibody
    • ErbB-3 binding protein 1 antibody
    • ErbB3 binding protein 1 antibody
    • ErbB3-binding protein 1 antibody
    • ErbB3-binding protein Ebp1 antibody
    • hG4 1 antibody
    • hG4-1 antibody
    • IRES-specific cellular trans-acting factor 45 kDa antibody
    • MGC81621 antibody
    • MGC94070 antibody
    • Mpp1 antibody
    • p38 2G4 antibody
    • Pa2g4 antibody
    • PA2G4_HUMAN antibody
    • Plfap antibody
    • Proliferation associated 2G4 antibody
    • Proliferation associated 2G4, 38-KD antibody
    • Proliferation-associated 2G4, 38kDa antibody
    • Proliferation-associated 2G4, a antibody
    • Proliferation-associated protein 1 antibody
    • Proliferation-associated protein 2G4 antibody
    • Protein p38-2G4 antibody
    • si:dz150i12.2 antibody
    • wu:fb19b11 antibody
    • wu:ft56d05 antibody
    • zgc:86732 antibody
    see all

Images

  • Western blot - Anti-EBP1 antibody (ab137566)
    Western blot - Anti-EBP1 antibody (ab137566)
    Anti-EBP1 antibody (ab137566) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 44 kDa



    10% SDS PAGE
  • Western blot - Anti-EBP1 antibody (ab137566)
    Western blot - Anti-EBP1 antibody (ab137566)
    Anti-EBP1 antibody (ab137566) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 44 kDa



    10% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody (ab137566)
    Immunocytochemistry/ Immunofluorescence - Anti-EBP1 antibody (ab137566)
    Immunofluorescence analysis of methanol fixed A431 cells, labelling EBP1 with ab137566 at 1/200 dilution (left), costained with Hoechst 33342 (right).

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab137566? Please let us know so that we can cite the reference in this datasheet.

    ab137566 has not yet been referenced specifically in any publications.

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