Key features and details
- Rabbit polyclonal to EBP1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-EBP1 antibody
See all EBP1 primary antibodies
DescriptionRabbit polyclonal to EBP1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human EBP1. The exact sequence is proprietary.
Database link: Q9UQ80
- Extracts of mouse liver, BXPC3, Hela, PC12, NIH 3T3, K562, 22RV1 and HT29 cell lines.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/500 - 1/2000. Predicted molecular weight: 44 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionMay play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site).
Tissue specificityExpressed in several cell lines tested, including primary and transformed cell lines.
Sequence similaritiesBelongs to the peptidase M24 family.
modificationsPhosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent.
Cellular localizationCytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG.
- Information by UniProt
- 38kDa antibody
- AA672939 antibody
- Cell cycle protein p38 2G4 homolog antibody
All lanes : Anti-EBP1 antibody (ab186846) at 1/500 dilution
Lane 1 : Extract of mouse liver cell line
Lane 2 : Extract of BXPC3 cell line
Lane 3 : Extract of Hela cell line
Lane 4 : Extract of PC12 cell line
Lane 5 : Extract of NIH 3T3 cell line
Lane 6 : Extract of K562 cell line
Lane 7 : Extract of 22RV1 cell line
Lane 8 : Extract of HT29 cell line
Predicted band size: 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach cancer tissue labelling EBP1 with ab186846 at 1/200. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186846. Blue DAPI for nuclear staining.
ab186846 has been referenced in 1 publication.
- Hwang I & Ahn JY Dysregulation of Epigenetic Control Contributes to Schizophrenia-Like Behavior in Ebp1+/- Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32283721