Overview

  • Product name

  • Description

    Rabbit polyclonal to EBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human EBP1. The exact sequence is proprietary.
    Database link: Q9UQ80

  • Positive control

    • Extracts of mouse liver, BXPC3, Hela, PC12, NIH 3T3, K562, 22RV1 and HT29 cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab186846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 44 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function

    May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site).
  • Tissue specificity

    Expressed in several cell lines tested, including primary and transformed cell lines.
  • Sequence similarities

    Belongs to the peptidase M24 family.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG.
  • Information by UniProt
  • Database links

  • Alternative names

    • 38kDa antibody
    • AA672939 antibody
    • Cell cycle protein p38 2G4 homolog antibody
    • Cell cycle protein p38-2G4 homolog antibody
    • ErbB-3 binding protein 1 antibody
    • ErbB3 binding protein 1 antibody
    • ErbB3-binding protein 1 antibody
    • ErbB3-binding protein Ebp1 antibody
    • hG4 1 antibody
    • hG4-1 antibody
    • IRES-specific cellular trans-acting factor 45 kDa antibody
    • MGC81621 antibody
    • MGC94070 antibody
    • Mpp1 antibody
    • p38 2G4 antibody
    • Pa2g4 antibody
    • PA2G4_HUMAN antibody
    • Plfap antibody
    • Proliferation associated 2G4 antibody
    • Proliferation associated 2G4, 38-KD antibody
    • Proliferation-associated 2G4, 38kDa antibody
    • Proliferation-associated 2G4, a antibody
    • Proliferation-associated protein 1 antibody
    • Proliferation-associated protein 2G4 antibody
    • Protein p38-2G4 antibody
    • si:dz150i12.2 antibody
    • wu:fb19b11 antibody
    • wu:ft56d05 antibody
    • zgc:86732 antibody
    see all

Images

  • All lanes : Anti-EBP1 antibody (ab186846) at 1/500 dilution

    Lane 1 : Extract of mouse liver cell line
    Lane 2 : Extract of BXPC3 cell line
    Lane 3 : Extract of Hela cell line
    Lane 4 : Extract of PC12 cell line
    Lane 5 : Extract of NIH 3T3 cell line
    Lane 6 : Extract of K562 cell line
    Lane 7 : Extract of 22RV1 cell line
    Lane 8 : Extract of HT29 cell line

    Predicted band size: 44 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach cancer tissue labelling EBP1 with ab186846 at 1/200. Magnification: 400x.
  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186846. Blue DAPI for nuclear staining.

References

ab186846 has not yet been referenced specifically in any publications.

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