Anti-EBP1 antibody (ab186846)
Key features and details
- Rabbit polyclonal to EBP1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-EBP1 antibody
See all EBP1 primary antibodies -
Description
Rabbit polyclonal to EBP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human EBP1. The exact sequence is proprietary.
Database link: Q9UQ80 -
Positive control
- Extracts of mouse liver, BXPC3, Hela, PC12, NIH 3T3, K562, 22RV1 and HT29 cell lines.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Predicted molecular weight: 44 kDa.
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IHC-P |
1/50 - 1/200.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 44 kDa. |
IHC-P
1/50 - 1/200. |
Target
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Function
May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). -
Tissue specificity
Expressed in several cell lines tested, including primary and transformed cell lines. -
Sequence similarities
Belongs to the peptidase M24 family. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG. - Information by UniProt
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Database links
- Entrez Gene: 5036 Human
- Entrez Gene: 18813 Mouse
- Entrez Gene: 288778 Rat
- Omim: 602145 Human
- SwissProt: Q9UQ80 Human
- SwissProt: P50580 Mouse
- Unigene: 524498 Human
- Unigene: 573018 Human
see all -
Alternative names
- 38kDa antibody
- AA672939 antibody
- Cell cycle protein p38 2G4 homolog antibody
see all
Images
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All lanes : Anti-EBP1 antibody (ab186846) at 1/500 dilution
Lane 1 : Extract of mouse liver cell line
Lane 2 : Extract of BXPC3 cell line
Lane 3 : Extract of Hela cell line
Lane 4 : Extract of PC12 cell line
Lane 5 : Extract of NIH 3T3 cell line
Lane 6 : Extract of K562 cell line
Lane 7 : Extract of 22RV1 cell line
Lane 8 : Extract of HT29 cell line
Predicted band size: 44 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach cancer tissue labelling EBP1 with ab186846 at 1/200. Magnification: 400x.
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186846. Blue DAPI for nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab186846 has been referenced in 1 publication.
- Hwang I & Ahn JY Dysregulation of Epigenetic Control Contributes to Schizophrenia-Like Behavior in Ebp1+/- Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32283721