Overview

  • Product name

  • Description

    Rabbit polyclonal to EBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human EBP1 aa 300-345. The exact sequence is proprietary. (NP_006182.2 ).
    Database link: Q9UQ80

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab225713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site).
  • Tissue specificity

    Expressed in several cell lines tested, including primary and transformed cell lines.
  • Sequence similarities

    Belongs to the peptidase M24 family.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG.
  • Information by UniProt
  • Database links

  • Alternative names

    • 38kDa antibody
    • AA672939 antibody
    • Cell cycle protein p38 2G4 homolog antibody
    • Cell cycle protein p38-2G4 homolog antibody
    • ErbB-3 binding protein 1 antibody
    • ErbB3 binding protein 1 antibody
    • ErbB3-binding protein 1 antibody
    • ErbB3-binding protein Ebp1 antibody
    • hG4 1 antibody
    • hG4-1 antibody
    • IRES-specific cellular trans-acting factor 45 kDa antibody
    • MGC81621 antibody
    • MGC94070 antibody
    • Mpp1 antibody
    • p38 2G4 antibody
    • Pa2g4 antibody
    • PA2G4_HUMAN antibody
    • Plfap antibody
    • Proliferation associated 2G4 antibody
    • Proliferation associated 2G4, 38-KD antibody
    • Proliferation-associated 2G4, 38kDa antibody
    • Proliferation-associated 2G4, a antibody
    • Proliferation-associated protein 1 antibody
    • Proliferation-associated protein 2G4 antibody
    • Protein p38-2G4 antibody
    • si:dz150i12.2 antibody
    • wu:fb19b11 antibody
    • wu:ft56d05 antibody
    • zgc:86732 antibody
    see all

Images

  • All lanes : Anti-EBP1 antibody (ab225713) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Exposure time: 30 seconds
  • Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for EBP1 using ab225713 at 1/200 dilution in immunohistochemical analysis.

    Detection: DAB staining. Counterstain: hematoxylin.

  • EBP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225713 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225713 at 1 µg/ml.

    Lane 1: ab225713 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.


    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab225713 has not yet been referenced specifically in any publications.

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