The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Detects a band of approximately 41 kDa (predicted molecular weight: 44 kDa). We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10x less for ECL detection.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site).
Expressed in several cell lines tested, including primary and transformed cell lines.
Belongs to the peptidase M24 family.
Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent.
Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG.