Recombinant Anti-EBP1 antibody [EPR14568(B)] (ab180603)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14568(B)] to EBP1
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-EBP1 antibody [EPR14568(B)]
See all EBP1 primary antibodies -
Description
Rabbit monoclonal [EPR14568(B)] to EBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T, Jurkat and A431 lysates; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14568(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).
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Flow Cyt (Intra) |
1/20.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa). |
Flow Cyt (Intra)
1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). -
Tissue specificity
Expressed in several cell lines tested, including primary and transformed cell lines. -
Sequence similarities
Belongs to the peptidase M24 family. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG. - Information by UniProt
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Database links
- Entrez Gene: 5036 Human
- Entrez Gene: 18813 Mouse
- Entrez Gene: 288778 Rat
- Omim: 602145 Human
- SwissProt: Q9UQ80 Human
- SwissProt: P50580 Mouse
- Unigene: 524498 Human
- Unigene: 573018 Human
see all -
Alternative names
- 38kDa antibody
- AA672939 antibody
- Cell cycle protein p38 2G4 homolog antibody
see all
Images
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedJurkat cells labeling EBP1 with ab180603 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green),followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.
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All lanes : Anti-EBP1 antibody [EPR14568(B)] (ab180603) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab180603 has not yet been referenced specifically in any publications.