Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14569(B)] to EBP1
- Suitable for: IHC-P, WB, IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-EBP1 antibody [EPR14569(B)]
See all EBP1 primary antibodies
DescriptionRabbit monoclonal [EPR14569(B)] to EBP1
Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EBP1 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UQ80
- 293T, HeLa, Jurkat and A431 lysates; Human colon adenocarcinoma tissue; HeLa cells; Jurkat cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site).
Tissue specificityExpressed in several cell lines tested, including primary and transformed cell lines.
Sequence similaritiesBelongs to the peptidase M24 family.
modificationsPhosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent.
Cellular localizationCytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG.
- Information by UniProt
- 38kDa antibody
- AA672939 antibody
- Cell cycle protein p38 2G4 homolog antibody
All lanes : Anti-EBP1 antibody [EPR14569(B)] (ab180602) at 1/20000 dilution
Lane 1 : 293T lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lane 4 : A431 lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling EBP1 with 180602 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (Left panel). Right panel: Dapi staining.
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling EBP1 with ab180602 at 1/100 dilution, followed by prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling EBP1 with ab180602 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.
Western blot analysis of Jurkat lysate immunoprecipitated with ab180602 at 1/30 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.
ab180602 has not yet been referenced specifically in any publications.