Overview

  • Product name

    Anti-EBP1 antibody [EPR14569(B)]
    See all EBP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14569(B)] to EBP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IP, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EBP1 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UQ80

  • Positive control

    • 293T, HeLa, Jurkat and A431 lysates; Human colon adenocarcinoma tissue; HeLa cells; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).
IP 1/30.
ICC/IF 1/100.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site).
  • Tissue specificity

    Expressed in several cell lines tested, including primary and transformed cell lines.
  • Sequence similarities

    Belongs to the peptidase M24 family.
  • Post-translational
    modifications

    Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Tranlocates to the nucleus upon treatment with HRG.
  • Information by UniProt
  • Database links

  • Alternative names

    • 38kDa antibody
    • AA672939 antibody
    • Cell cycle protein p38 2G4 homolog antibody
    • Cell cycle protein p38-2G4 homolog antibody
    • ErbB-3 binding protein 1 antibody
    • ErbB3 binding protein 1 antibody
    • ErbB3-binding protein 1 antibody
    • ErbB3-binding protein Ebp1 antibody
    • hG4 1 antibody
    • hG4-1 antibody
    • IRES-specific cellular trans-acting factor 45 kDa antibody
    • MGC81621 antibody
    • MGC94070 antibody
    • Mpp1 antibody
    • p38 2G4 antibody
    • Pa2g4 antibody
    • PA2G4_HUMAN antibody
    • Plfap antibody
    • Proliferation associated 2G4 antibody
    • Proliferation associated 2G4, 38-KD antibody
    • Proliferation-associated 2G4, 38kDa antibody
    • Proliferation-associated 2G4, a antibody
    • Proliferation-associated protein 1 antibody
    • Proliferation-associated protein 2G4 antibody
    • Protein p38-2G4 antibody
    • si:dz150i12.2 antibody
    • wu:fb19b11 antibody
    • wu:ft56d05 antibody
    • zgc:86732 antibody
    see all

Images

  • All lanes : Anti-EBP1 antibody [EPR14569(B)] (ab180602) at 1/20000 dilution

    Lane 1 : 293T lysate
    Lane 2 : HeLa lysate
    Lane 3 : Jurkat lysate
    Lane 4 : A431 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling EBP1 with 180602 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (Left panel). Right panel: Dapi staining.

  • Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling EBP1 with ab180602 at 1/100 dilution, followed by prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling EBP1 with ab180602 at 1/20 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Western blot analysis of Jurkat lysate immunoprecipitated with ab180602 at 1/30 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.

References

ab180602 has not yet been referenced specifically in any publications.

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