Overview

  • Product name

    Anti-EBP50/NHERF-1 antibody
    See all EBP50/NHERF-1 primary antibodies
  • Description

    Rabbit polyclonal to EBP50/NHERF-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human EBP50/NHERF-1 aa 275-325. The exact sequence is proprietary. (NP_004243.1).
    Database link: O14745

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IHC-P: Human breast carcinoma tissue. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab225904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 38 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.
  • Tissue specificity

    Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.
  • Involvement in disease

    Defects in SLC9A3R1 are the cause of hypophosphatemic nephrolithiasis/osteoporosis type 2 (NPHLOP2) [MIM:612287]. Hypophosphatemia results from idiopathic renal phosphate loss. It contributes to the pathogenesis of hypophosphatemic urolithiasis (formation of urinary calculi) as well to that of hypophosphatemic osteoporosis (bone demineralization).
  • Sequence similarities

    Contains 2 PDZ (DHR) domains.
  • Post-translational
    modifications

    Phosphorylated on serine residues.
  • Cellular localization

    Cytoplasm. Apical cell membrane. Endomembrane system. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus. Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner (By similarity). Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Found in microvilli, ruffling membrane and filopodia of HeLa cells. Present in lipid rafts of T-cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • EBP 50 antibody
    • EBP50 antibody
    • Ezrin radixin moesin binding phosphoprotein 50 antibody
    • Ezrin-radixin-moesin-binding phosphoprotein 50 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE RF antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE-RF1 antibody
    • Na+/H+ exchange regulatory co factor antibody
    • NHERF 1 antibody
    • NHERF antibody
    • NHERF-1 antibody
    • NHERF1 antibody
    • NHRF1_HUMAN antibody
    • NPHLOP2 antibody
    • Regulatory cofactor of Na(+)/H(+) exchanger antibody
    • Slc9a3r1 antibody
    • Sodium hydrogen exchanger regulatory factor 1 antibody
    • Sodium-hydrogen exchanger regulatory factor 1 antibody
    • Sodium/hydrogen exchanger regulatory factor 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform 3 regulatory factor 1 antibody
    • Solute carrier family 9 isoform A3 regulatory factor 1 antibody
    • Solute carrier family 9 member 3 regulator 1 antibody
    see all

Images

  • All lanes : Anti-EBP50/NHERF-1 antibody (ab225904) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 38 kDa


    Exposure time: 3 minutes
  • Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for EBP50/NHERF-1 using ab225904 at 1/200 dilution in immunohistochemical analysis.

    Detection: DAB staining.

  • EBP50/NHERF-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225904 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225904 at 1 µg/ml.

    Lane 1: ab225904 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 seconds.

References

ab225904 has not yet been referenced specifically in any publications.

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