Anti-EBV Latent Membrane Protein 1 antibody [CS1, CS2, CS3, CS4], prediluted (ab7502)

Overview

  • Product name
    Anti-EBV Latent Membrane Protein 1 antibody [CS1, CS2, CS3, CS4], prediluted
    See all EBV Latent Membrane Protein 1 primary antibodies
  • Description
    Mouse monoclonal [CS1, CS2, CS3, CS4] to EBV Latent Membrane Protein 1, prediluted
  • Host species
    Mouse
  • Specificity
    This antibody is specific to 60 kD latent membrane protein (LMP1) encoded by the BNLF1 gene of the EBV. Each clone reacts with different epitopes on the hydrophilic c-terminus of the cytoplasmic domain of LMP1. This antibody stains strongly with EBV positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis.
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    EBV.
  • Immunogen

    Recombinant fusion protein containing sequences of bacterial beta-galactosidase and EBV encoded Latent membrane protein.

  • Positive control
    • Infected lymphoblastoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab7502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    EBV is a human herpesvirus that establishes a life-long persistence in the host. The virus infects the vast majority of the world's adult population and is well known for its association with a broad spectrum of benign and malignant diseases, including infectious mononucleosis, Burkitt's lymphoma, nasopharyngeal carcinoma, and is causally associated with lymphoid and epithelial malignancies, including post-transplant lymphoproliferative disorders, Hodgkin's disease, anaplastic nasopharyngeal carcinoma and gastric carcinomas. Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is a transforming protein that affects multiple cell signalling pathways and contributes to EBV-associated oncogenesis. LMP1 can be expressed in some states of EBV latency, and significant induction of full-length LMP1 is also observed frequently during virus reactivation into the lytic cycle. LMP1 is critical for EBV-infected cell activation, adhesion and survival, and is usually expressed in the malignant cells.
  • Alternative names
    • BNLF1 antibody
    • EBV antibody
    • Epstein Barr virus antibody
    • HHV4 antibody
    • Human Herpesvirus 4 antibody
    • Latent membrane protein 1 antibody
    • LMP1 antibody
    see all

Images

  • The image shows lung tissue diagnosed with Post-Transplantation Lymphoproliferative Disorder (PTLD). Mononuclear cells are stained brown with DAB chromogen around the cytoplasm - typical for Latent Membrane Protein-1 of EBV.

    This picture was kindly supplied as part of the review submitted by Pradeep Rai.

References

This product has been referenced in:
  • Nawandar DM  et al. Differentiation-Dependent LMP1 Expression Is Required for Efficient Lytic Epstein-Barr Virus Reactivation in Epithelial Cells. J Virol 91:N/A (2017). Read more (PubMed: 28179525) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

What was your order number? I do not see any orders to Peru in my records.

The IHC-P image on the datasheet is from a customer's testing. The image shows lung tissue diagnosed with Post-Transplantation Lymphoproliferative Disorder (PTLD). Mononuclear cells are stained brown with DAB chromogen around the cytoplasm - typical for Latent Membrane Protein-1 of EBV. You can see his basic protocol linked below and you can contact him by clicking on his name online.

https://www.abcam.com/index.html?datasheet=7502&tab=abreviews&intabreviewid=2103

I have some references listed here from the lab, but I'm unsure if they are background articles or cite this antibody in particultar: The lab recommends infected lymphoblastoma as a positive control, and the references are below.

1) Jarrett et al. J Clin Pathol 44: 844, 1991.
2) Murray et al. J Pathol 166:1, 1992.
3) Pallesen et al. Lancet 337: 320, 1991.
4) Rowe et al. J Gene Virol 68: 1575, 1987.

The lab also recommends following this protocol:

http://www.jove.com/pdf/308/jove-protocol-308-immunohistochemistry-paraffin-sections-using-vectastain-abc-kit-from?ID=308

Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

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Answer

Thank you for contacting us. This antibody is non-hazardous. Please find the requested MSDS for non-hazardous products attached. Let me know if you need any additional information or assistance.

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Question
Answer

Thank you for your enquiry. Unfortunately this information was not released by the originator of the antibody. I am sorry I could not be more helpful. Please do not hesitate to contact us if you need anything further.

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Question
Answer

The approximate concentration of ab7502 is 8 ug/ml.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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