Overview

  • Product name
    Anti-EBV Nuclear Antigen antibody [E1-2.5]
    See all EBV Nuclear Antigen primary antibodies
  • Description
    Mouse monoclonal [E1-2.5] to EBV Nuclear Antigen
  • Host species
    Mouse
  • Specificity
    Recognizes repetitive gly-ala region of the nuclear antigen 1 of Epstein Barr virus (EBNA-1).
  • Tested applications
    Suitable for: ICC, ELISA, IHC-Pmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    E1-2.5
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
ELISA
IHC-P
  • Application notes
    ELISA: Use at an assay dependant concentration.
    ICC: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is the one EBV antigen that is expressed in all EBV associated malignancies. It has long been thought to go undetected by the cell mediated immune system. However, recent studies show that EBNA1 can be presented to both CD4+ and CD8+ T cells, making it a potential new target for immunotherapy of EBV related cancers.
    • Cellular localization
      Nuclear. Free in the nucleoplasm, somewhat associated with the chromatin and hardly, if at all associated with the nuclear matrix.
    • Alternative names
      • EBNA1 antibody
      • EBV nuclear antigen 1 antibody
      • Epstein Barr nuclear antigen 1 antibody
      • Epstein Barr virus antibody
      • HHV4 antibody
      • Human Herpesvirus 4 antibody
      see all

    Images

    • ab8329 staining EBV Nuclear Antigen in Human breast ductal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in 10mM citrate buffer. Samples were blocked with 3% hydrogen peroxide in methanol for 5 minutes. Samples were incubated with primary antibody (1/1000 in 0.5M Tris hydrochloride, pH 7.6) for 30 minutes at room temperature.

    References

    This product has been referenced in:
    • Wang H  et al. The Hsp70 inhibitor 2-phenylethynesulfonamide inhibits replication and carcinogenicity of Epstein-Barr virus by inhibiting the molecular chaperone function of Hsp70. Cell Death Dis 9:734 (2018). Read more (PubMed: 29959331) »
    • Ni C  et al. In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures. Cell Res 25:785-800 (2015). Read more (PubMed: 25916549) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Answer

    Thank you for your enquiry. I am afraid that I have been unable to find out further details with regards the immunogen used for ab8329. I will endeavour to get back in touch with the source of this antibody, but at present they have no further information. With respect to ab25653; a recombinant GST-EBNA1 (459-641aa) was used as the antigen. Unfortunately the epitope has not been mapped for this antibody. I am sorry that I cannot be of more assistance at this point in time.

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    Answer

    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in Western blotting. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). We do have another EBNA1 antibody that has been tested for application in Western blotting, it is ab25653, Mouse monoclonal [1B5] to EBNA1. Please contact us again if you have any additional questions.

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    Question
    Answer

    Thank you for your enquiry. I have been in touch with the source of this antibody and unfortunately they were unable to provide me with specific epitope details. However, details of the specificity for this antibody are such that it recognizes the repetitive gly-ala region. Examination of the Swiss-Prott entry P03211 located at the following link suggests that there is a repeating core unit of AGAGG which increases to AGAGGG after several repeats and is eventually interspersed with AGG repeats. Given that the epitope resides within this repeat region it is likely to have strong cross reactivity against different regions of the repeat region on account of the similarity of the sequence. Therefore it is highly likely that the antibody would recognise an 8 amino acid sequence that is comprised of the repeat core unit AGAGG. However, the exact sequence that the antibody recognizes has not been fully determined. http://ca.expasy.org/cgi-bin/sprot-ft-details.pl?P03211@COMPBIAS@87@352 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Answer

    Thank you for your enquiry. Yes, ab6786 - Goat polyclonal to Mouse IgG H&L (rhodamine) - would be a suitable secondary antibody to use with ab8329 - Mouse monoclonal [E1-2.5] to EBNA 1. The list of compatible secondaries located at the bottom of the datasheets are just suggestions, and don't encompass all of the secondary antibodies that we have that can be used with that particular primary antibody. If you have any additional questions, please let us know.

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    Answer

    Thank you for your enquiry and your interest in our products. According to the datasheet, this antibody has been tested for ELISA and ICC applications but we do not have any information if it works for Western blot. We would suggest that you should carry out a preliminary experiment with different dilutions of this antibody (i.e.1:100-1:2000) and then adjust the dilution accordingly from the results obtained.

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    Answer

    This antibody has not yet been tested for use with paraffin embedded tissue. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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