Anti-EBV Nuclear Antigen antibody [E1-2.5] (ab8329)


  • Product name
    Anti-EBV Nuclear Antigen antibody [E1-2.5]
    See all EBV Nuclear Antigen primary antibodies
  • Description
    Mouse monoclonal [E1-2.5] to EBV Nuclear Antigen
  • Host species
  • Specificity
    Recognizes repetitive gly-ala region of the nuclear antigen 1 of Epstein Barr virus (EBNA-1).
  • Tested applications
    Suitable for: ICC, ELISA, IHC-Pmore details


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab8329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependant concentration.
    ICC: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is the one EBV antigen that is expressed in all EBV associated malignancies. It has long been thought to go undetected by the cell mediated immune system. However, recent studies show that EBNA1 can be presented to both CD4+ and CD8+ T cells, making it a potential new target for immunotherapy of EBV related cancers.
    • Cellular localization
      Nuclear. Free in the nucleoplasm, somewhat associated with the chromatin and hardly, if at all associated with the nuclear matrix.
    • Alternative names
      • EBNA1 antibody
      • EBV nuclear antigen 1 antibody
      • Epstein Barr nuclear antigen 1 antibody
      • Epstein Barr virus antibody
      • HHV4 antibody
      • Human Herpesvirus 4 antibody
      see all


    • ab8329 staining EBV Nuclear Antigen in Human breast ductal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in 10mM citrate buffer. Samples were blocked with 3% hydrogen peroxide in methanol for 5 minutes. Samples were incubated with primary antibody (1/1000 in 0.5M Tris hydrochloride, pH 7.6) for 30 minutes at room temperature.


    This product has been referenced in:
    • Ni C  et al. In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures. Cell Res 25:785-800 (2015). Read more (PubMed: 25916549) »
    • Cardinaud S  et al. The synthesis of truncated polypeptides for immune surveillance and viral evasion. PLoS One 5:e8692 (2010). Read more (PubMed: 20098683) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry. I am afraid that I have been unable to find out further details with regards the immunogen used for ab8329. I will endeavour to get back in touch with the source of this antibody, but at present they have no further info...

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    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in Western blotting. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get...

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    Thank you for your enquiry. I have been in touch with the source of this antibody and unfortunately they were unable to provide me with specific epitope details. However, details of the specificity for this antibody are such that it recognizes the ...

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    Thank you for your enquiry. Yes, ab6786 - Goat polyclonal to Mouse IgG H&L (rhodamine) - would be a suitable secondary antibody to use with ab8329 - Mouse monoclonal [E1-2.5] to EBNA 1. The list of compatible secondaries located at the bottom of the...

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    Thank you for your enquiry and your interest in our products. According to the datasheet, this antibody has been tested for ELISA and ICC applications but we do not have any information if it works for Western blot. We would suggest that you sho...

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    This antibody has not yet been tested for use with paraffin embedded tissue. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.


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