• Product name

    Anti-EBV Nuclear Antigen/EBNA1 antibody [E1-2.5]
    See all EBV Nuclear Antigen/EBNA1 primary antibodies
  • Description

    Mouse monoclonal [E1-2.5] to EBV Nuclear Antigen/EBNA1
  • Host species

  • Specificity

    Recognizes repetitive gly-ala region of the nuclear antigen 1 of Epstein Barr virus (EBNA-1).
  • Tested applications

    Suitable for: ICC, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Other species
  • General notes

     This product was previously labelled as EBV Nuclear Antigen



  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab8329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    ELISA: Use at an assay dependant concentration.
    ICC: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is the one EBV antigen that is expressed in all EBV associated malignancies. It has long been thought to go undetected by the cell mediated immune system. However, recent studies show that EBNA1 can be presented to both CD4+ and CD8+ T cells, making it a potential new target for immunotherapy of EBV related cancers.
    • Cellular localization

      Nuclear. Free in the nucleoplasm, somewhat associated with the chromatin and hardly, if at all associated with the nuclear matrix.
    • Alternative names

      • EBNA1 antibody
      • EBV nuclear antigen 1 antibody
      • Epstein Barr nuclear antigen 1 antibody
      • Epstein Barr virus antibody
      • HHV4 antibody
      • Human Herpesvirus 4 antibody
      see all


    • ab8329 staining EBV Nuclear Antigen/EBNA1 in Human breast ductal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in 10mM citrate buffer. Samples were blocked with 3% hydrogen peroxide in methanol for 5 minutes. Samples were incubated with primary antibody (1/1000 in 0.5M Tris hydrochloride, pH 7.6) for 30 minutes at room temperature.


    This product has been referenced in:

    • Wang H  et al. The Hsp70 inhibitor 2-phenylethynesulfonamide inhibits replication and carcinogenicity of Epstein-Barr virus by inhibiting the molecular chaperone function of Hsp70. Cell Death Dis 9:734 (2018). Read more (PubMed: 29959331) »
    • Wang C  et al. Berberine inhibits the proliferation of human nasopharyngeal carcinoma cells via an Epstein-Barr virus nuclear antigen 1-dependent mechanism. Oncol Rep 37:2109-2120 (2017). Read more (PubMed: 28259949) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A


    Thank you for your enquiry. I am afraid that I have been unable to find out further details with regards the immunogen used for ab8329. I will endeavour to get back in touch with the source of this antibody, but at present they have no further information. With respect to ab25653; a recombinant GST-EBNA1 (459-641aa) was used as the antigen. Unfortunately the epitope has not been mapped for this antibody. I am sorry that I cannot be of more assistance at this point in time.

    Read More


    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in Western blotting. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). We do have another EBNA1 antibody that has been tested for application in Western blotting, it is ab25653, Mouse monoclonal [1B5] to EBNA1. Please contact us again if you have any additional questions.

    Read More


    Thank you for your enquiry. I have been in touch with the source of this antibody and unfortunately they were unable to provide me with specific epitope details. However, details of the specificity for this antibody are such that it recognizes the repetitive gly-ala region. Examination of the Swiss-Prott entry P03211 located at the following link suggests that there is a repeating core unit of AGAGG which increases to AGAGGG after several repeats and is eventually interspersed with AGG repeats. Given that the epitope resides within this repeat region it is likely to have strong cross reactivity against different regions of the repeat region on account of the similarity of the sequence. Therefore it is highly likely that the antibody would recognise an 8 amino acid sequence that is comprised of the repeat core unit AGAGG. However, the exact sequence that the antibody recognizes has not been fully determined. http://ca.expasy.org/cgi-bin/sprot-ft-details.pl?P03211@COMPBIAS@87@352 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More


    Thank you for your enquiry. Yes, ab6786 - Goat polyclonal to Mouse IgG H&L (rhodamine) - would be a suitable secondary antibody to use with ab8329 - Mouse monoclonal [E1-2.5] to EBNA 1. The list of compatible secondaries located at the bottom of the datasheets are just suggestions, and don't encompass all of the secondary antibodies that we have that can be used with that particular primary antibody. If you have any additional questions, please let us know.

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    Thank you for your enquiry and your interest in our products. According to the datasheet, this antibody has been tested for ELISA and ICC applications but we do not have any information if it works for Western blot. We would suggest that you should carry out a preliminary experiment with different dilutions of this antibody (i.e.1:100-1:2000) and then adjust the dilution accordingly from the results obtained.

    Read More


    This antibody has not yet been tested for use with paraffin embedded tissue. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

    Read More

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