Product nameAnti-EBV Nuclear Antigen/EBNA1 antibody [E1-2.5]
See all EBV Nuclear Antigen/EBNA1 primary antibodies
DescriptionMouse monoclonal [E1-2.5] to EBV Nuclear Antigen/EBNA1
SpecificityRecognizes repetitive gly-ala region of the nuclear antigen 1 of Epstein Barr virus (EBNA-1).
Tested applicationsSuitable for: ICC, ELISA, IHC-Pmore details
Species reactivityReacts with: Other species
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab8329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use at an assay dependant dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceEpstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is the one EBV antigen that is expressed in all EBV associated malignancies. It has long been thought to go undetected by the cell mediated immune system. However, recent studies show that EBNA1 can be presented to both CD4+ and CD8+ T cells, making it a potential new target for immunotherapy of EBV related cancers.
Cellular localizationNuclear. Free in the nucleoplasm, somewhat associated with the chromatin and hardly, if at all associated with the nuclear matrix.
- EBNA1 antibody
- EBV nuclear antigen 1 antibody
- Epstein Barr nuclear antigen 1 antibody
ab8329 staining EBV Nuclear Antigen/EBNA1 in Human breast ductal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in 10mM citrate buffer. Samples were blocked with 3% hydrogen peroxide in methanol for 5 minutes. Samples were incubated with primary antibody (1/1000 in 0.5M Tris hydrochloride, pH 7.6) for 30 minutes at room temperature.
This product has been referenced in:
- Wang H et al. The Hsp70 inhibitor 2-phenylethynesulfonamide inhibits replication and carcinogenicity of Epstein-Barr virus by inhibiting the molecular chaperone function of Hsp70. Cell Death Dis 9:734 (2018). Read more (PubMed: 29959331) »
- Wang C et al. Berberine inhibits the proliferation of human nasopharyngeal carcinoma cells via an Epstein-Barr virus nuclear antigen 1-dependent mechanism. Oncol Rep 37:2109-2120 (2017). Read more (PubMed: 28259949) »