Key features and details
- Rabbit polyclonal to ECE1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ECE1 antibody
DescriptionRabbit polyclonal to ECE1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Cow, Xenopus laevis
Synthetic peptide corresponding to Human ECE1 aa 700 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: HUVEC MEF1 NIH 3T3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab71829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 87 kDa).|
RelevanceFunction: Converts big endothelin-1 to endothelin-1. Tissue specificity: All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Disease: Hirschsprung disease cardiac defects and autonomic dysfunction Similarity: Belongs to the peptidase M13 family.
Cellular localizationCell Membrane. Single pass type II membrane protein.
- ECE 1 antibody
- ECE antibody
- ECE-1 antibody
All lanes : Anti-ECE1 antibody (ab71829) at 1 µg/ml
Lane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
ECE1 contains 10 predicted glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab71829 has been referenced in 2 publications.
- Ghodrati F et al. The prion protein is embedded in a molecular environment that modulates transforming growth factor ß and integrin signaling. Sci Rep 8:8654 (2018). WB ; Human . PubMed: 29872131
- Padilla J et al. TRAF3IP2 mediates high glucose-induced endothelin-1 production as well as endothelin-1-induced inflammation in endothelial cells. Am J Physiol Heart Circ Physiol 314:H52-H64 (2018). PubMed: 28971844