Product nameAnti-ECE1 antibody
DescriptionRabbit polyclonal to ECE1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Cow, Xenopus laevis
Synthetic peptide corresponding to Human ECE1 aa 700 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: HUVEC MEF1 NIH 3T3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab71829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 87 kDa).|
RelevanceFunction: Converts big endothelin-1 to endothelin-1. Tissue specificity: All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney. Disease: Hirschsprung disease cardiac defects and autonomic dysfunction Similarity: Belongs to the peptidase M13 family.
Cellular localizationCell Membrane. Single pass type II membrane protein.
- ECE 1 antibody
- ECE antibody
- ECE-1 antibody
All lanes : Anti-ECE1 antibody (ab71829) at 1 µg/ml
Lane 1 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
ECE1 contains 10 predicted glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
This product has been referenced in:
- Ghodrati F et al. The prion protein is embedded in a molecular environment that modulates transforming growth factor ß and integrin signaling. Sci Rep 8:8654 (2018). WB ; Human . Read more (PubMed: 29872131) »
- Padilla J et al. TRAF3IP2 mediates high glucose-induced endothelin-1 production as well as endothelin-1-induced inflammation in endothelial cells. Am J Physiol Heart Circ Physiol 314:H52-H64 (2018). Read more (PubMed: 28971844) »